Rothamsted Research, Harpenden, Herts, UK.
Pest Manag Sci. 2014 Apr;70(4):610-4. doi: 10.1002/ps.3589. Epub 2013 Jul 4.
Traps suitable for catching female Agriotes click beetles may provide better reconnaissance than pheromone-baited traps which catch only males, thereby contributing to more efficient crop protection. The basis for this study came from (i) observations of female Agriotes brevis (Candeze) aggregating beneath foliage of Medicago sativa (L.) and Lolium italicum (A. Br.) placed on plastic sheets on bare soil, and (ii) field tests demonstrating attraction of females to traps baited with foliage from these plants. The aim was to identify and field test volatile compounds from M. sativa and L. italicum leaves.
A number of electrophysiologically active chemicals were identified from headspace extracts of M. sativa and L. italicum. Three different synthetic blends of the identified compounds, comprising four, seven and nine components, were field tested. The four- and nine-component blends caught more female A. brevis than unbaited traps, with the proportion of females not differing between blends.
The plant-derived blends were shown to catch female A. brevis under field conditions when applied in traps. Of these, the four-component blend, given its relatively simple composition [(Z)-3-hexenyl acetate:methyl benzoate:(Z)-3-hexen-1-ol:methyl salicylate 300:5:30:30 mg bait(-1)], may be a suitable 'standard' blend for bait optimisation.
适用于捕捉雌性 Agriotes 叩甲的陷阱可能比仅捕捉雄性的性信息素诱饵陷阱提供更好的侦察效果，从而有助于更有效地进行作物保护。本研究的基础来自于（i）观察到雌性 Agriotes brevis（Candeze）在放在裸露土壤上的塑料片上的 Medicago sativa（L.）和 Lolium italicum（A. Br.）的叶子下聚集，以及（ii）田间试验证明雌性对这些植物的叶子诱饵的吸引力。目的是鉴定和田间测试来自 Medicago sativa 和 Lolium italicum 叶子的挥发性化合物。
从 Medicago sativa 和 Lolium italicum 的顶空提取物中鉴定出许多电生理活性化学物质。三种不同的鉴定化合物的合成混合物，由四个、七个和九个成分组成，进行了田间测试。四成分和九成分混合物比未诱饵的陷阱捕捉到更多的雌性 A. brevis，而混合成分之间的雌性比例没有差异。
在田间条件下，当应用于陷阱时，植物衍生的混合物显示出可以捕捉雌性 A. brevis。其中，四成分混合物（（Z）-3-己烯基乙酸酯：苯甲酸甲酯：（Z）-3-己烯-1-醇：水杨酸甲酯 300：5：30：30 mg 诱饵（-1）），由于其相对简单的组成，可能是一种合适的“标准”混合物，用于优化诱饵。
