胃腺癌中波形蛋白表达和 Her-2 状态的临床意义。
Clinical significance of vimentin expression and Her-2 status in patients with gastric carcinoma.
机构信息
Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, China.
出版信息
Clin Transl Sci. 2013 Jun;6(3):184-90. doi: 10.1111/cts.12043. Epub 2013 Apr 19.
OBJECTIVES
To determine whether vimentin could be used as a marker of gastric carcinomas with more aggressive behavior. To detect the extent of Her-2 status in gastric carcinoma and explore the correlation between vimentin expression and Her-2 status.
METHODS
Vimentin expression was detected in surgically resected gastric carcinoma tissue specimens from 143 patients by immunohistochemistry. The human epidermal growth factor receptor 2 (Her2) status was evaluated by fluorescence in situ hybridization (FISH). Correlations between vimentin expression, Her-2 status and clinicopathological factors were evaluated using Kaplan-Meier method and Cox multivariate survival models.
RESULTS
Vimentin expression was significantly correlated with age, advanced stage, poorly differentiated type, venous invasion, hepatic metastasis, and recurrence (p < 0.05). Her-2 gene was amplified in 16 (11.2%) out of the 143 gastric carcinoma tissue specimens. Her-2 status was correlated with advanced cancer, poor differentiation, venous invasion, hepatic metastasis, and recurrence (p < 0.05). The result of multivariate analysis showed that vimentin expression and lymph node metastasis were independent prognostic factors.
CONCLUSIONS
Vimentin expression in epithelial cells of the surgically resected gastric adenocarcinoma tissue is an independent predictor of short survival, and Her-2 status shows a valuable correlation with clinical parameters.
目的
确定波形蛋白是否可作为具有侵袭性行为的胃癌的标志物。检测胃癌中 Her-2 状态的程度，并探讨波形蛋白表达与 Her-2 状态之间的相关性。
方法
采用免疫组织化学法检测 143 例手术切除的胃癌组织标本中波形蛋白的表达。采用荧光原位杂交（FISH）评估人表皮生长因子受体 2（Her2）状态。采用 Kaplan-Meier 法和 Cox 多因素生存模型评估波形蛋白表达、Her-2 状态与临床病理因素之间的相关性。
结果
波形蛋白表达与年龄、晚期、低分化、静脉侵犯、肝转移和复发显著相关（p<0.05）。143 例胃癌组织标本中，有 16 例（11.2%）Her-2 基因扩增。Her-2 状态与癌症晚期、分化差、静脉侵犯、肝转移和复发相关（p<0.05）。多因素分析结果表明，波形蛋白表达和淋巴结转移是独立的预后因素。
结论
手术切除的胃腺癌组织中上皮细胞的波形蛋白表达是总生存期短的独立预测因子，而 Her-2 状态与临床参数具有有价值的相关性。
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