Mirzamani Monireh Sadat, Nourani Mohammad Reza, Imani Fooladi Abbas Ali, Zare Samad, Ebrahimi Majid, Yazdani Samaneh, Ghanei Mostafa, Karimfar Mohammad Hassan
Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran, and Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran.
Iran J Allergy Asthma Immunol. 2013 May 15;12(2):144-52.
The widespread use of sulfur mustard (SM) as a chemical warfare agent in the past century has proved its long-lasting toxic effects. Despite a lot of research over the past decades on Iranian veterans, there are still major gaps in the SM literature. Transforming growth factor (TGF-β), a cytokine that affects many different cell processes, has an important role in the lungs of patients with some of chronic airway diseases, especially with respect to airway remodeling in mustard lung. Primary airway fibroblasts from epibronchial biopsies were cultured, and gene expression of TGF-β1, TGF-β2, TbR-I and TbR-II in fibroblasts of SM injured patients and controls were investigated. Expression of TGF-βs and receptors was measured by RT-PCR. Protein level of TGF-β1 was surveyed by western blot. Our findings revealed that expression levels of TGF-β1, TGF-β2, TbR-I and TbR-II were upregulated in the airway fibroblasts of SM exposed patients in comparison with control samples. TGF-β1 expression was shown to be markedly increased in primary lung fibroblasts of chemically injured patients. Our novel data, suggested that over-expression of TGF-β molecule and receptors in primary airway fibroblasts of mustard gas injured patients may be involved in progression of airway remodeling of these patients.
在过去的一个世纪里，硫芥作为一种化学战剂被广泛使用，这证明了它具有持久的毒性作用。尽管在过去几十年里对伊朗退伍军人进行了大量研究，但硫芥相关文献仍存在重大空白。转化生长因子（TGF-β）是一种影响许多不同细胞过程的细胞因子，在一些慢性气道疾病患者的肺部中发挥着重要作用，尤其是在芥子气肺气道重塑方面。培养来自支气管活检的原代气道成纤维细胞，研究硫芥损伤患者和对照组成纤维细胞中TGF-β1、TGF-β2、TbR-I和TbR-II的基因表达。通过RT-PCR检测TGF-βs和受体的表达。通过蛋白质印迹法检测TGF-β1的蛋白水平。我们的研究结果显示，与对照样本相比，硫芥暴露患者气道成纤维细胞中TGF-β1、TGF-β2、TbR-I和TbR-II的表达水平上调。化学损伤患者的原代肺成纤维细胞中TGF-β1表达明显增加。我们的新数据表明，芥子气损伤患者原代气道成纤维细胞中TGF-β分子和受体的过度表达可能与这些患者气道重塑的进展有关。
