基质辅助激光解吸电离飞行时间质谱分析芯片。
Analysis of microarrays by MALDI-TOF MS.
机构信息
CICbiomaGUNE, Biofunctional Nanomaterials Unit, Paseo Miramon 182, 20009 San Sebastian, Spain.
出版信息
Angew Chem Int Ed Engl. 2013 Jul 15;52(29):7477-81. doi: 10.1002/anie.201302455. Epub 2013 Jun 11.
PMID:23757366
Abstract
Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.
摘要
配体文库可以打印到三明治结构上，该三明治由嵌入在疏水性层中的活化脂质和与铟锡氧化物（ITO）表面相连的疏水性层组成。通过这种方式产生的阵列可以通过荧光光谱法和质谱法进行分析。该方法的应用包括酶特异性分配、糖型分析和凝集素鉴定。
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