Greve H, Blumberg P, Schmidt G, Schlumberger W, Rauterberg J, Kresse H
Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Federal Republic of Germany.
Biochem J. 1990 Jul 1;269(1):149-55. doi: 10.1042/bj2690149.
Small dermatan sulphate proteoglycan II from cultured human skin fibroblasts interacts with type I collagen in vitro and in vivo. When fibroblasts are maintained in a type I collagen lattice the proteoglycan remains exclusively within the lattice, and its association with fibrils can be demonstrated immunocytochemically. On the basis of [35S]sulphate incorporation, small proteoglycan II comprises about 80% of total proteoglycans secreted by cells in monolayer culture. In a collagen lattice, fibroblasts down-regulate its synthesis to the level of large chondroitin sulphate/dermatan sulphate and of heparan sulphate proteoglycans, the synthesis of which remains unaffected. Compared with the product from monolayer cultures, small proteoglycan II from collagen gels contained a longer polysaccharide chain which is characterized by a larger proportion of disulphated and a smaller proportion of monosulphated glucuronic acid-containing disaccharides. The half-life varied between 60 and 110 h. It is suggested that the compositional differences between the proteoglycan from monolayer cultures and from cells in a collagen lattice are related to the slower intracellular trafficking of the proteoglycan under the latter culture conditions.
来自培养的人皮肤成纤维细胞的小分子硫酸皮肤素蛋白聚糖II在体外和体内均与I型胶原相互作用。当成纤维细胞维持在I型胶原晶格中时，蛋白聚糖仅保留在晶格内，并且其与纤维的结合可以通过免疫细胞化学方法证实。根据[35S]硫酸盐掺入情况，小分子蛋白聚糖II约占单层培养细胞分泌的总蛋白聚糖的80%。在胶原晶格中，成纤维细胞将其合成下调至大硫酸软骨素/硫酸皮肤素和硫酸乙酰肝素蛋白聚糖的水平，而后者的合成不受影响。与单层培养产物相比，来自胶原凝胶的小分子蛋白聚糖II含有更长的多糖链，其特征在于含双硫酸化的二糖比例更大，含单硫酸化葡萄糖醛酸的二糖比例更小。半衰期在60至110小时之间变化。有人提出，单层培养的蛋白聚糖与胶原晶格中细胞的蛋白聚糖之间的组成差异与后者培养条件下蛋白聚糖较慢的细胞内运输有关。
