大鼠白色和棕色脂肪细胞中A1型腺苷受体特性的差异。
Differences in the properties of A1-type adenosine receptors in rat white and brown adipocytes.
作者信息
Saggerson E D, Jamal Z
机构信息
Department of Biochemistry, University College London, U.K.
出版信息
Biochem J. 1990 Jul 1;269(1):157-61. doi: 10.1042/bj2690157.
Abstract
- White adipocytes were found to be more responsive than brown adipocytes to inhibition of lipolysis by the A1 adenosine receptor agonist phenylisopropyladenosine. 2. Radioligand binding studies with plasma membranes isolated from the two adipocyte types indicated differences in the properties of the A1 receptors. Kd values (high and low affinity) for phenylisopropyladenosine were higher in membranes from brown adipocytes. The Kd values for the antagonist dipropylcyclopentylxanthine were also higher in brown adipocyte membranes. 3. The effects of guanine nucleotides in converting adipocyte A1 receptors into a low-affinity state were enhanced by dithiothreitol.
摘要
- 研究发现，白色脂肪细胞比棕色脂肪细胞对A1腺苷受体激动剂苯异丙基腺苷抑制脂肪分解的作用更敏感。2. 对从两种脂肪细胞类型中分离出的质膜进行放射性配体结合研究，结果表明A1受体的特性存在差异。棕色脂肪细胞质膜中苯异丙基腺苷的解离常数（高亲和力和低亲和力）更高。拮抗剂二丙基环戊基黄嘌呤的解离常数在棕色脂肪细胞质膜中也更高。3. 二硫苏糖醇增强了鸟嘌呤核苷酸将脂肪细胞A1受体转化为低亲和力状态的作用。
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