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人类滑膜细胞分泌一种39 kDa的蛋白质，该蛋白质类似于牛在非泌乳期表达的乳腺蛋白。

Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period.

作者信息

Nyirkos P, Golds E E

机构信息

Shriners Hospital, Joint Diseases Laboratory, Montreal, Quebec, Canada.

出版信息

Biochem J. 1990 Jul 1;269(1):265-8. doi: 10.1042/bj2690265.

DOI:10.1042/bj2690265
PMID:2375755
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1131563/
Abstract

By SDS/PAGE analysis we have observed that human synovial cell monolayers secrete a prominent 39 kDa protein which could not be detected in skin and lung fibroblasts. This protein was purified to homogeneity by heparin-Sepharose chromatography and reverse-phase h.p.l.c. The N-terminal sequence was found to be almost identical to that of a recently described bovine protein detected in the mammary secretions during the involutionary phase of the lactational cycle. Characterization of this 39 kDa protein may provide a useful marker for classification of connective tissue cells.

摘要

通过SDS/PAGE分析，我们观察到人类滑膜细胞单层分泌一种显著的39 kDa蛋白质，而在皮肤和肺成纤维细胞中未检测到这种蛋白质。该蛋白质通过肝素-琼脂糖层析和反相高效液相色谱法纯化至同质。发现其N端序列与最近描述的一种在泌乳周期退化阶段乳腺分泌物中检测到的牛蛋白质的序列几乎相同。对这种39 kDa蛋白质的特性进行表征可能为结缔组织细胞的分类提供一个有用的标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da79/1131563/62431e537a5a/biochemj00180-0255-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da79/1131563/0e4798a839f9/biochemj00180-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da79/1131563/62431e537a5a/biochemj00180-0255-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da79/1131563/0e4798a839f9/biochemj00180-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da79/1131563/62431e537a5a/biochemj00180-0255-b.jpg

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本文引用的文献

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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
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Genetics. 2023 Feb 9;223(2). doi: 10.1093/genetics/iyac185.
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Chitinases and Chitinase-Like Proteins in Obstructive Lung Diseases - Current Concepts and Potential Applications.几丁质酶和几丁质酶样蛋白在阻塞性肺疾病中的作用——当前概念和潜在应用。
Int J Chron Obstruct Pulmon Dis. 2020 Apr 23;15:885-899. doi: 10.2147/COPD.S236640. eCollection 2020.
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Identification of Chitinase-3-Like Protein 1 as a Novel Neutrophil Antigenic Target in Crohn's Disease.鉴定壳三糖酶蛋白 1 为克罗恩病中性粒细胞的新型抗原性靶标。
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