Faculty of Veterinary Medicine, Department of Pathology, University of Mehmet Akif Ersoy, Istiklal Yerleskesi, Burdur, Turkey.
J Environ Pathol Toxicol Oncol. 2013;32(1):53-8. doi: 10.1615/jenvironpatholtoxicoloncol.2013006476.
Cardiovascular disease is one of the most significant causes of mortality in humans and animals, and its etiology is usually unknown. The aim of this study was to investigate the cardiac pathology of endosulfan toxicity and the protective effect of vitamin C in rabbits. Twenty-four rabbits were divided into 4 groups: (1) the END group was given a daily sublethal dose of endosulfan in corn oil by oral gavage for 6 weeks; (2) the END + C group received the endosulfan as well as vitamin C over the same 6-week period; (3) the OIL + C group received corn oil daily and vitamin C every other day; and (4) the OIL group received only corn oil daily. We observed microscopic hemorrhages, single-cell necrosis, inflammatory reactions, and fibrotic changes in the myocardium in the END group. Small hemorrhages and single-cell necrosis also were seen in some hearts in the END + C group, but no inflammation was observed. Caspase-3 immunoreactivity was more significant in myocardial cells in the END group compared with the others. A protective effect of vitamin C on lesions was observed in the END + C group. These results showed that endosulfan resulted in toxic changes in the hearts of rabbits, but this toxicity could be decreased with vitamin C treatment.
心血管疾病是人类和动物死亡的主要原因之一，其病因通常未知。本研究旨在探讨硫丹毒性对心脏的影响以及维生素 C 的保护作用。24 只兔子被分为 4 组：（1）END 组，每日通过口服玉米油给予亚致死剂量的硫丹，持续 6 周；（2）END+C 组，在相同的 6 周内接受硫丹和维生素 C；（3）OIL+C 组，每日接受玉米油，每隔一天接受维生素 C；（4）OIL 组，每日仅接受玉米油。我们观察到 END 组心肌中有微观出血、单细胞坏死、炎症反应和纤维化改变。在 END+C 组的一些心脏中也观察到小出血和单细胞坏死，但未观察到炎症。与其他组相比，END 组心肌细胞中的 caspase-3 免疫反应更明显。在 END+C 组中观察到维生素 C 对病变的保护作用。这些结果表明，硫丹导致兔子心脏产生毒性变化，但维生素 C 治疗可降低这种毒性。
