Ozmen O, Mor F
Department of Pathology, University of Mehmet Akif Ersoy, Faculty of Veterinary Medicine , 15030, Istiklal Yerleskesi, Burdur , Turkey.
Biotech Histochem. 2015 Jan;90(1):25-30. doi: 10.3109/10520295.2014.936507. Epub 2014 Aug 25.
Endosulfan is an insecticide that is composed of two stereoisomers: α- and β- endosulfan in an approximate ratio of 70:30. Owing to its widespread use, poisoning of both humans and animals is possible. We examined the toxic effects of endosulfan on New Zealand white rabbit kidneys. Rabbit kidneys were examined histopathologically and caspase-3 activity was detected using immunohistochemistry. Animals were divided into four groups: Group 1 was given a sublethal dose of endosulfan in corn oil by oral gavage daily for 6 weeks, Group 2 was given endosulfan + vitamin C during the same period, Group 3 was given corn oil daily and vitamin C on alternate days, Group 4 was given only corn oil daily throughout the experiment. By the end of experimental period, the concentration of α-endosulfan was greater than the β-endosulfan concentration in the kidneys of both of endosulfan treated groups (Groups 1 and 2). Decreased accumulation of α- and β-endosulfan was observed in Group 2, possibly because of the antioxidant effect of the vitamin C. Histopathological examination revealed hemorrhages, tubule cell necrosis, glomerular infiltration, glomerulosclerosis and proteinaceous material in the tubules, and Bowman spaces in the kidneys of Group 1. Caspase-3 reaction was stronger in Group 1 than in the other groups. Apoptotic activity was most frequent in proximal tubule cells. Endosulfan is toxic to rabbit kidneys. Vitamin C treatment reduced the accumulation of endosulfan in kidneys and reduced its toxicity.
α-硫丹和β-硫丹，其比例约为70:30。由于其广泛使用，人和动物都有可能中毒。我们研究了硫丹对新西兰白兔肾脏的毒性作用。对兔肾进行了组织病理学检查，并使用免疫组织化学检测了半胱天冬酶-3的活性。动物分为四组：第1组每天经口灌胃给予亚致死剂量的硫丹玉米油溶液，持续6周；第2组在同一时期给予硫丹+维生素C；第3组每天给予玉米油，隔天给予维生素C；第4组在整个实验期间仅每天给予玉米油。到实验期结束时，两个硫丹处理组（第1组和第2组）肾脏中的α-硫丹浓度均高于β-硫丹浓度。第2组中α-硫丹和β-硫丹的积累减少，可能是由于维生素C的抗氧化作用。组织病理学检查显示，第1组兔肾有出血、肾小管细胞坏死、肾小球浸润、肾小球硬化以及肾小管和鲍曼间隙中有蛋白质物质。第1组中的半胱天冬酶-3反应比其他组更强。凋亡活性在近端肾小管细胞中最为常见。硫丹对兔肾有毒性。维生素C处理可减少硫丹在肾脏中的积累并降低其毒性。
