Su Chun-xia, Duan Xiang-guo, Liang Li-jun, Zheng Jie, Fu Xue-Yan, Yan Ya-mei, Wang Ning-ping
School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
Laboratory Examination College, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China; Laboratory Examination Research Institute, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China; Stem Cell Research Institute, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
Vet Immunol Immunopathol. 2014 Mar 15;158(1-2):98-104. doi: 10.1016/j.vetimm.2013.05.006. Epub 2013 May 10.
Lycium barbarum polysaccharides (LBP) have been shown to play a variety of immune-modulatory functions which include activation of T and B cells. Follicular helper T (Tfh) cells are now recognized as a subset of helper T cells which regulate the multiple stages of B cell maturation and function. In our current study, we found that LBP were able to activate CXCR5+PD-1+ Tfh cells and induce IL-21 secretion. At the same time LBP also promoted the formation of germinal centers (GC) and production of GL-7+B220+GC B cells. LBP as an adjuvant could increase generation of rAd5VP1-induced Tfh cells. Our results indicate that LBP might enhance T cell-dependent Ab responses by acting as an adjuvant for the generation of Tfh cells. Future vaccine designs might therefore be targeted to induce strong antigen-specific Tfh responses in order to boost its protective effects.
枸杞多糖（LBP）已被证明具有多种免疫调节功能，包括激活T细胞和B细胞。滤泡辅助性T（Tfh）细胞现在被认为是辅助性T细胞的一个亚群，可调节B细胞成熟和功能的多个阶段。在我们目前的研究中，我们发现LBP能够激活CXCR5+PD-1+Tfh细胞并诱导IL-21分泌。同时，LBP还促进生发中心（GC）的形成以及GL-7+B220+GC B细胞的产生。LBP作为佐剂可增加重组腺病毒5型病毒蛋白1（rAd5VP1）诱导的Tfh细胞的生成。我们的结果表明，LBP可能通过作为Tfh细胞生成的佐剂来增强T细胞依赖性抗体反应。因此，未来的疫苗设计可能旨在诱导强烈的抗原特异性Tfh反应，以增强其保护作用。
