Zurich-Basel Plant Science Center; Department of Environmental Sciences, Botany; University of Basel; Basel, Switzerland.
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.25229. Epub 2013 Jun 7.
We have recently identified two genes coding for ammonium transporters (AMT) in Sorghum bicolor that were induced in roots colonized by arbuscular mycorrhizal (AM) fungi. To improve our understanding of the dynamics of ammonium transport in this symbiosis, we studied the transfer of soil-ammonium-derived (15)N to S. bicolor plants via the Glomus mosseae fungal mycelium in compartmented microcosms. The (15)NH (4+)-containing hyphal compartment was inaccessible to the roots in the plant compartment. (15)N label concentrations significantly increased in plant roots and leaves already 48 h after exposure of the AM fungus to the (15)NH (4+) substrate, attesting an efficient symbiotic N transfer between the symbiotic partners and further highlighting that AM symbiosis represents an important component of plant nitrogen nutrition.
我们最近在高粱中鉴定了两个编码氨转运体（AMT）的基因，这些基因在被丛枝菌根（AM）真菌定殖的根中被诱导。为了更好地理解这种共生关系中氨转运的动态，我们通过分隔的微宇宙研究了土壤铵衍生的（15）N 通过 Glomus mosseae 真菌菌丝向高粱植物的转移。植物隔室中的根无法进入含有（15）NH（4+）的菌丝隔室。在 AM 真菌暴露于（15）NH（4+）底物后 48 小时，植物根部和叶片中的（15）N 标记浓度显著增加，证明了共生伙伴之间有效的共生 N 转移，进一步强调了 AM 共生关系是植物氮素营养的重要组成部分。
