Agroécologie, INRAE, Institut Agro, Univ. Bourgogne, Univ. Bourgogne Franche-Comté, Dijon, France.
Institute of Botany, Czech Academy of Sciences, Průhonice, Czech Republic.
Plant Cell Environ. 2022 Nov;45(11):3354-3366. doi: 10.1111/pce.14426. Epub 2022 Sep 8.
Sorghum is an important worldwide source of food, feed and fibres. Like most plants, it forms mutualistic symbioses with arbuscular mycorrhizal fungi (AMF), but the nutritional basis of mycorrhiza-responsiveness is largely unknown. Here, we investigated the transcriptional and physiological responses of sorghum to two different AMF species, Rhizophagus irregularis and Funneliformis mosseae, under 16 different conditions of nitrogen (N) and phosphorus (P) supply. Our experiment reveals fine-scale differences between two AMF species in the nutritional interactions with sorghum plants. Physiological and gene expression patterns (ammonium transporters: AMT; phosphate transporters: PHT) indicate the existence of generalist or specialist mycorrhizal pathway. While R. irregularis switched on the mycorrhizal pathway independently of the plant nutritional status, F. mosseae influenced the mycorrhizal pathway depending on the N-to-P plant ratio and soil supply. The differences between both AMF species suggest some AMT and PHT as ideal candidates to develop markers for improving efficiency of nutrient acquisition in sorghum under P and N limitation, and for the selection of plant genotypes.
高粱是世界范围内重要的粮食、饲料和纤维来源。与大多数植物一样，它与丛枝菌根真菌（AMF）形成互利共生关系，但 AMF 响应的营养基础在很大程度上尚不清楚。在这里，我们研究了高粱对两种不同 AMF 物种——不规则丛枝菌根真菌（Rhizophagus irregularis）和摩西管柄囊霉（Funneliformis mosseae）——在 16 种不同氮（N）和磷（P）供应条件下的转录和生理响应。我们的实验揭示了两种 AMF 物种与高粱植物之间在营养相互作用方面的细微差异。生理和基因表达模式（铵转运蛋白：AMT；磷酸盐转运蛋白：PHT）表明存在一般性或专门性的菌根途径。不规则丛枝菌根真菌独立于植物营养状况开启菌根途径，而摩西管柄囊霉则根据 N 与 P 的植物比值和土壤供应影响菌根途径。两种 AMF 物种之间的差异表明，一些 AMT 和 PHT 可以作为理想的候选标记，用于提高高粱在 P 和 N 限制下的养分获取效率，并用于植物基因型的选择。
