Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, United States.
Syst Appl Microbiol. 2013 Sep;36(6):376-82. doi: 10.1016/j.syapm.2013.04.002. Epub 2013 Jun 5.
Members of the phylum Fibrobacteres are highly efficient cellulolytic bacteria, best known for their role in rumen function and as potential sources of novel enzymes for bioenergy applications. Despite being key members of ruminants and other digestive microbial communities, our knowledge of this phylum remains incomplete, as much of our understanding is focused on two recognized species, Fibrobacter succinogenes and F. intestinalis. As a result, we lack insights regarding the environmental niche, host range, and phylogenetic organization of this phylum. Here, we analyzed over 1000 16S rRNA Fibrobacteres sequences available from public databases to establish a phylogenetic framework for this phylum. We identify both species- and genus-level clades that are suggestive of previously unknown taxonomic relationships between Fibrobacteres in addition to their putative lifestyles as host-associated or free-living. Our results shed light on this poorly understood phylum and will be useful for elucidating the function, distribution, and diversity of these bacteria in their niches.
纤维杆菌门的成员是高效的纤维素分解菌，以其在反刍动物功能中的作用以及作为生物能源应用中新型酶的潜在来源而闻名。尽管它们是反刍动物和其他消化微生物群落的主要成员，但我们对该门的了解仍不完整，因为我们的大部分理解都集中在两个公认的物种，纤维杆菌 succinogenes 和 F. intestinalis 上。因此，我们缺乏关于该门的环境生态位、宿主范围和系统发育组织的信息。在这里，我们分析了来自公共数据库的 1000 多个 16S rRNA 纤维杆菌序列，以建立该门的系统发育框架。我们确定了种和属水平的进化枝，这表明纤维杆菌之间存在以前未知的分类关系，除了它们作为宿主相关或自由生活的潜在生活方式。我们的结果揭示了这个了解甚少的门，并将有助于阐明这些细菌在其生态位中的功能、分布和多样性。
