在必需核糖体蛋白L3和S10中引入的单个突变会导致枯草芽孢杆菌出现孢子形成缺陷。

Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis.

作者信息

Akanuma Genki, Suzuki Shota, Yano Koichi, Nanamiya Hideaki, Natori Yousuke, Namba Eri, Watanabe Kazuya, Tagami Kazumi, Takeda Takuya, Iizuka Yuka, Kobayashi Ako, Ishizuka Morio, Yoshikawa Hirofumi, Kawamura Fujio

机构信息

Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo 112-8551, Japan.

出版信息

J Gen Appl Microbiol. 2013;59(2):105-17. doi: 10.2323/jgam.59.105.

DOI:10.2323/jgam.59.105

PMID:23759864

Abstract

We introduced single mutations into the rplC and rpsJ genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium Bacillus subtilis, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45°C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.

摘要

我们在rplC和rpsJ基因中引入了单突变，这两个基因分别编码必需的核糖体蛋白L3（RplC）和S10（RpsJ），它们位于革兰氏阳性、形成芽孢的细菌枯草芽孢杆菌的S10基因簇中，并研究了这些突变是否影响其生长速率、孢子形成、感受态发育和70S核糖体形成。在位于50S肽基转移酶中心的L3核糖体蛋白中携带G52D突变的突变细胞，在45°C时积累30S亚基，这可能是由于50S形成缺陷所致，并且在高温下孢子形成频率降低。另一方面，在位于30S氨酰-tRNA位点附近的S10蛋白中携带H56R突变的突变细胞，表现出严重的生长缺陷和孢子形成缺陷，并且在感受态发育方面也出现显著延迟。