Korea Food Research Institute, 1201 Anyangpangyoro, Bundang-gu, Seongnam-si, Gyeonggi-do 463-746, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:921012. doi: 10.1155/2013/921012. Epub 2013 May 22.
The antidiabetic effect of the Citrus junos Tanaka (also known as yuja or yuzu) was examined. Ethanol extract of yuja peel (YPEE) significantly stimulated 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) uptake in C2C12 myotubes. However, ethanol extract of yuja pulp (YpEE) and water extract of yuja peel (YPWE) or pulp (YpWE) did not stimulate glucose uptake. In addition, peroxisome proliferator-activated receptor gamma (PPAR-γ) and AMP-activated protein kinase (AMPK) activities were increased by YPEE in a dose-dependent manner. Pretreatment of AMPK inhibitor decreased the glucose uptake stimulated by YPEE in C2C12 myotubes. We confirmed the anti-diabetic effect of YPEE in mice fed a high fat-diet (HFD). Compared with control mice on a normal diet (ND), these mice showed increased body weight, liver fat, insulin resistance, triacylglycerol (TG), and total cholesterol content. Addition of 5% YPEE significantly reduced the weight gain and rise in liver fat content, serum triacylglycerol (TG), total cholesterol, and insulin resistance found in mice fed a high-fat diet (HFD). Moreover, YPEE reduced the secretion of HFD-induced adipocytokines such as leptin and resistin. YPEE also resulted in increased phosphorylation of AMPK in muscle tissues. These results suggest that ethanol extract of yuja peel exerts anti-diabetic effects via AMPK and PPAR-γ in both cell culture and mouse models.
研究了柑橘柚（也称为柚子或柚子）的抗糖尿病作用。柚子皮的乙醇提取物（YPEE）可显着刺激 C2C12 肌管中 2-[N-（7-硝基苯并-2-氧代-1,3-二唑-4-基）氨基]-2-脱氧-D-葡萄糖（2-NBDG）的摄取。然而，柚子果肉的乙醇提取物（YpEE）和柚子皮的水提取物（YPWE）或果肉（YpWE）均不能刺激葡萄糖摄取。此外，YPEE 以剂量依赖性方式增加过氧化物酶体增殖物激活受体γ（PPAR-γ）和 AMP 激活的蛋白激酶（AMPK）活性。AMPK 抑制剂预处理可降低 YPEE 在 C2C12 肌管中刺激的葡萄糖摄取。我们在高脂饮食（HFD）喂养的小鼠中证实了 YPEE 的抗糖尿病作用。与正常饮食（ND）对照组相比，这些小鼠的体重，肝脂肪，胰岛素抵抗，三酰基甘油（TG）和总胆固醇含量均增加。添加 5％YPEE 可显着减少高脂饮食（HFD）喂养的小鼠的体重增加和肝脂肪含量升高，血清三酰基甘油（TG），总胆固醇和胰岛素抵抗。此外，YPEE 减少了 HFD 诱导的脂肪细胞因子（如瘦素和抵抗素）的分泌。YPEE 还导致肌肉组织中 AMPK 的磷酸化增加。这些结果表明，柚子皮的乙醇提取物通过细胞培养和小鼠模型中的 AMPK 和 PPAR-γ发挥抗糖尿病作用。
