Abe Hiroko, Ishioka Mika, Fujita Yasuko, Umeno Aya, Yasunaga Mayu, Sato Akihiko, Ohnishi Shigehiko, Suzuki Shingo, Ishida Noriko, Shichiri Mototada, Yoshida Yasukazu, Nakajima Yoshihiro
Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST).
Shikoku Research Institute Inc.
J Oleo Sci. 2018 Mar 1;67(3):335-344. doi: 10.5650/jos.ess17184. Epub 2018 Feb 19.
Ulcerative colitis is a well-known inflammatory bowel disease. Although there are drugs that are effective against this disease, the prevention and attenuation of ulcerative colitis by food rich in functional ingredients without side effects is desired because some drugs have side effects. In this study, we investigated the effects of yuzu (Citrus junos Tanaka), a citrus fruit native to northeast Asia, on a mouse dextran sulfate sodium (DSS)-induced colitis model. Mice given drinking water containing DSS showed significant weight loss, colon shortening, diarrhea, and visible fecal blood. In contrast, mice fed a diet containing 5% yuzu peel for 14 d before receiving DSS showed significant attenuation of these phenotypes. To clarify the mechanism underlying the attenuation, we investigated the anti-inflammatory and antioxidant effects of yuzu peel. We found that yuzu peel extract suppressed tumor necrosis factor-α (TNF-α) production in lipopolysaccharide (LPS)-stimulated mice and murine macrophage cell line through suppression of nuclear factor-κB (NF-κB) activation. In addition, we confirmed that yuzu peel extract had a moderate antioxidant effect. These results suggest that yuzu peel attenuates the pathologies of DSS-induced colitis by coordinately suppressing inflammation and oxidative stress against lipids in vivo.
溃疡性结肠炎是一种广为人知的炎症性肠病。尽管有药物可有效治疗这种疾病，但由于某些药物有副作用，人们期望通过富含功能成分且无副作用的食物来预防和减轻溃疡性结肠炎。在本研究中，我们调查了原产于亚洲东北部的柑橘类水果柚子（Citrus junos Tanaka）对小鼠葡聚糖硫酸钠（DSS）诱导的结肠炎模型的影响。饮用含DSS的水的小鼠出现显著体重减轻、结肠缩短、腹泻以及肉眼可见的粪便带血。相比之下，在接受DSS之前喂食含5%柚子皮饮食14天的小鼠，这些表型得到显著减轻。为阐明减轻作用的潜在机制，我们研究了柚子皮的抗炎和抗氧化作用。我们发现柚子皮提取物通过抑制核因子-κB（NF-κB）激活，抑制脂多糖（LPS）刺激的小鼠和小鼠巨噬细胞系中肿瘤坏死因子-α（TNF-α）的产生。此外，我们证实柚子皮提取物具有适度的抗氧化作用。这些结果表明，柚子皮通过在体内协同抑制炎症和脂质氧化应激来减轻DSS诱导的结肠炎的病理变化。
