Keller L A M, Pereyra C M, Cavaglieri L R, Dalcero A M, Rosa C A R
Departamento de Microbiologia e Imunologia Veterinária, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465 Km 7, 23890-000 Seropédica, RJ, Brazil ; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brasilia, DF, Brazil.
ISRN Vet Sci. 2012 Oct 15;2012:396590. doi: 10.5402/2012/396590. Print 2012.
The aim of the study was to determine the mycobiota and natural levels of mycotoxins such as aflatoxin B1 (AFB1), ochratoxin A (OTA), fumonisin B1 (FB1), and deoxynivalenol (DON) present in brewers grains pre- and poststored intended for bovine intensive rearing. Poststored (80%) samples had counts higher than 1 × 10(4) colony-forming units (CFU/g). Cladosporium spp. and Aspergillus spp. were isolated at high frequencies. Aspergillus flavus was the prevalent isolated species. Prestored (70%) and poststored (100%) samples showed AFB1 levels over the recommended limits (20 μ g/Kg), and OTA levels were below the recommended limits (50 μ g/Kg) while pre- and poststored samples did not show FB1 and DON natural contamination levels. The presence of mycotoxins in this substrate indicates the existence of contamination. Regular monitoring of feeds is required in order to prevent chronic and acute toxic syndromes related to this kind of contamination.
本研究的目的是确定用于奶牛集约化饲养的储存前后啤酒糟中真菌群落以及黄曲霉毒素B1(AFB1)、赭曲霉毒素A(OTA)、伏马菌素B1(FB1)和脱氧雪腐镰刀菌烯醇(DON)等霉菌毒素的天然水平。储存后的(80%)样本菌落形成单位计数高于1×10⁴CFU/g。枝孢属和曲霉属的分离频率较高。黄曲霉是主要的分离菌种。储存前(70%)和储存后(100%)的样本中AFB1水平超过推荐限值(20μg/Kg),OTA水平低于推荐限值(50μg/Kg),而储存前后的样本均未显示FB1和DON的天然污染水平。该底物中霉菌毒素的存在表明存在污染。需要定期监测饲料,以预防与此类污染相关的慢性和急性中毒综合征。