从自然发生山羊乳腺炎病例的乳汁中分离和鉴定丝状支原体山羊亚种

Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases.

作者信息

Kumar Vijay, Rana Rajneesh, Mehra Somya, Rout Pramod Kumar

机构信息

Central Institute for Research on Goats, Makhdoom, P.O. Farah, Mathura 281122, Uttar Pradesh, India.

出版信息

ISRN Vet Sci. 2013 May 20;2013:593029. doi: 10.1155/2013/593029. Print 2013.

DOI:10.1155/2013/593029

PMID:23762593

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3673333/

Abstract

Association of Mycoplasma mycoides subspecies capri (Mmc) with natural goat mastitis has been studied earlier largely by detecting the Mmc DNA using molecular methods. However, report on detection of cultivable Mmc isolates from natural goat-mastitis milk is still very rare. In this study, Mmc was isolated from milk samples (n = 171) of goats with or without clinical signs of mastitis. Mmc isolates were further characterized by biochemical and species-specific PCR methods. Intra species strain variation was also studied by 16S amplified rDNA restriction analysis (16S ARDRA). The study recovered a total of 6 Mmc isolates (3.5%). Three types of intraspecies variants among the recovered Mmc isolates were found by 16S ARDRA. The study concluded that Mmc may be an etiological agent of mycoplasmal mastitis in Indian goat herds.

摘要

此前，主要通过分子方法检测丝状支原体山羊亚种（Mmc）DNA，对其与天然山羊乳腺炎的关联进行了大量研究。然而，关于从患有天然山羊乳腺炎的乳汁中分离出可培养的Mmc菌株的报道仍然非常罕见。在本研究中，从有或无乳腺炎临床症状的山羊乳汁样本（n = 171）中分离出了Mmc。通过生化和种特异性PCR方法对Mmc分离株进行了进一步鉴定。还通过16S扩增rDNA限制性分析（16S ARDRA）研究了种内菌株变异。该研究共分离出6株Mmc菌株（3.5%）。通过16S ARDRA在分离出的Mmc菌株中发现了三种种内变体。该研究得出结论，Mmc可能是印度山羊群中支原体性乳腺炎的病原体。