Isolation and molecular characterization of spp. in sheep and goats in Egypt.

BACKGROUND AND AIM

Different species of are associated with many pathological problems in small ruminants including respiratory manifestation, this problem results in significant losses, especially in African countries. This study aimed to (I) study some epidemiological aspects of species infections in Egyptian sheep and goats at Giza Governorate, (II) diagnosis of species affections using bacterial isolation and identification, (III) apply the polymerase chain reaction (PCR) for typing of different species, and (IV) illustrate the phylogenetic tree for the isolated species and other species from GenBank using the purified PCR product.

MATERIALS AND METHODS

A total of 335 samples were collected from sheep and goats from Giza Governorate in Egypt as 142 nasal swabs from clinically affected animals, 167 pneumonic lungs, 18 samples from tracheal bifurcation, and 8 samples by bronchial wash were cultured on pleuropneumonia-like organisms (PPLOs) media for cultivation of species. PCR and sequencing and phylogenetic analysis were adopted to identify and classify the isolated species.

RESULTS

A total of 24 isolates were isolated on PPLO media, identified by biochemical tests, and confirmed and typed by PCR using specific primers. 10 isolates were confirmed as , four isolates as by PCR, and 10 isolates as undifferentiated . A purified isolate of and was sequenced and phylogenetic analysis was illustrated.

CONCLUSION

and are prevalent in Egyptian sheep and goats. Further studies on are required due to its high frequency of isolation from pneumonic sheep and goats and also from animals suffer from different respiratory manifestations.