海南岛亚种HN-A的首次报道及比较基因组分析

First Report and Comparative Genomic Analysis of a Subspecies HN-A in Hainan Island.

作者信息

Zhang Zhenxing, Jiang Junming, He Meirong, Li Haoyang, Cheng Yiwen, An Qi, Chen Si, Du Li, Man Churiga, Chen Qiaoling, Li Lianbin, Wang Fengyang

机构信息

Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Engineering Key Laboratory of Haikou, College of Animal Science and Technology, Hainan University, Haikou 570228, China.

出版信息

Microorganisms. 2022 Sep 26;10(10):1908. doi: 10.3390/microorganisms10101908.

DOI:10.3390/microorganisms10101908

PMID:36296185

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9607973/

Abstract

subspecies (Mmc) is one of the six cluster (Mm cluster) members, which can cause "MAKePS" (Mastitis, Arthritis, Keratoconjunctivitis, Pneumonia, Septicemia) syndrome in ruminants. These symptoms can occur alone or together in individuals or flocks of goats. However, little is known about the epidemic Mmc strains in Hainan Island, China. We aimed to isolate the endemic Mmc strains in Hainan Island and reveal their molecular characteristics by genomic sequencing and comparative genomics to mitigate the impact of Mmc on local ruminant farming. Here, the Mmc HN-A strain was isolated and identified for the first time in Hainan Island, China. The genome of Mmc HN-A was sequenced. It contains a 1,084,691 bp-long circular chromosome and 848 coding genes. The genomic analysis of Mmc HN-A revealed 16 virulence factors, 2 gene islands, and a bacterial type IV secretion system protein VirD4. Comparative genomics showed that the core genome of the five contained 611 genes that could be exploited to develop drugs and endemic vaccines. Additionally, 36 specific genes were included in the Mmc HN-A genome, which could provide the possibility for the further control and prevention of the Mmc effects on local ruminants and enrich the information on Mmc strains.

摘要

亚种（Mmc）是六个聚类（Mm聚类）成员之一，可在反刍动物中引起“MAKePS”（乳腺炎、关节炎、角膜结膜炎、肺炎、败血症）综合征。这些症状可单独出现，也可在山羊个体或群体中同时出现。然而，关于中国海南岛流行的Mmc菌株知之甚少。我们旨在分离海南岛的地方性Mmc菌株，并通过基因组测序和比较基因组学揭示其分子特征，以减轻Mmc对当地反刍动物养殖的影响。在此，首次在中国海南岛分离并鉴定出Mmc HN-A菌株。对Mmc HN-A的基因组进行了测序。它包含一条1,084,691 bp长的环状染色体和848个编码基因。Mmc HN-A的基因组分析揭示了16个毒力因子、2个基因岛和一种细菌IV型分泌系统蛋白VirD4。比较基因组学表明，这五个菌株的核心基因组包含611个可用于开发药物和地方性疫苗的基因。此外，Mmc HN-A基因组中包含36个特定基因，这可为进一步控制和预防Mmc对当地反刍动物的影响提供可能性，并丰富有关Mmc菌株的信息。