Mechanism of cell rear retraction in migrating cells.

For decades, ever growing data on myosin II provides strong evidence that interaction of myosin-II-motor-domain with actin filaments within cells retracts the cell rear during actin-based cell migration. Now it is clear myosin II motor-activity is not the sole force involved. Alternative force-generating mechanisms within cells clearly also exist to power cell rear retraction during actin-based cell migration. Given that nematode sperm cells migrate without actin and without cytoskeletal motor proteins it is perhaps not surprising other types of force power cell rear retraction in actin-based systems. Here, cell rear retraction driven by actin filament depolymerisation, actin filament crosslinking, cell front protrusion and possibly apparent membrane tension and their importance relative to myosin II-motor-based contractility are discussed.