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工程屏障调控成骨细胞沿垂直方向迁移。

Engineered barriers regulate osteoblast cell migration in vertical direction.

机构信息

Department of Electrical Engineering, City University of Hong Kong, Kowloon, Hong Kong, China.

Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Kowloon, Hong Kong, China.

出版信息

Sci Rep. 2022 Mar 15;12(1):4459. doi: 10.1038/s41598-022-08262-5.

DOI:10.1038/s41598-022-08262-5
PMID:35292702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8924172/
Abstract

Considering cell migration is essential for understanding physiological processes and diseases. The vertical migration of cells in three dimensions is vital, but most previous studies on cell migration have only focused on two-dimensional horizontal migration. In this paper, cell migration in the vertical direction was studied. Barriers with a height of 1, 5, 10, and 25 µm with grating and arrows in channels as guiding patterns were fabricated. The effects of barrier height and guiding patterns on the vertical migration of MC3T3 cells were explored. The study revealed that taller barriers hinder vertical migration of MC3T3 cells, whereas grating and arrows in channels promote it. The time-lapse and micrograph images showed that as the barrier height increased, the cell climbing angle along the barrier sidewall decreased, and the time taken to climb over the barrier increased. These results indicate that taller barriers increase the difficulty of vertical migration by MC3T3 cells. To promote the vertical migration of MC3T3 cells, 10 µm tall barriers with 18° and 40° sloped sidewalls were fabricated. For barriers with 18° sloped sidewalls, the probability for MC3T3 cells to climb up and down the 10 µm tall barriers was 40.6% and 20.3%, respectively; this is much higher than the migration probability over vertical barriers. This study shows topographic guidance on the vertical migration of MC3T3 cells and broadens the understanding of cell migration in the vertical direction.

摘要

考虑到细胞迁移对于理解生理过程和疾病至关重要。细胞在三维方向上的垂直迁移至关重要，但之前大多数关于细胞迁移的研究仅集中在二维水平迁移上。本文研究了细胞在垂直方向上的迁移。制作了高度为 1、5、10 和 25 µm 的带有格栅和箭头的通道作为导向图案的障碍物。研究了障碍物高度和导向图案对 MC3T3 细胞垂直迁移的影响。研究表明，较高的障碍物会阻碍 MC3T3 细胞的垂直迁移，而通道中的格栅和箭头则会促进其迁移。延时和显微镜图像显示，随着障碍物高度的增加，细胞沿着障碍物侧壁的攀爬角度减小，越过障碍物所需的时间增加。这些结果表明，较高的障碍物会增加 MC3T3 细胞垂直迁移的难度。为了促进 MC3T3 细胞的垂直迁移，制作了高度为 10 µm 且具有 18°和 40°倾斜侧壁的障碍物。对于具有 18°倾斜侧壁的障碍物，MC3T3 细胞爬上和爬下 10 µm 高障碍物的概率分别为 40.6%和 20.3%；这远高于垂直障碍物上的迁移概率。本研究展示了 MC3T3 细胞垂直迁移的地形导向，并拓宽了对细胞在垂直方向上迁移的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/01b0f0b2e37d/41598_2022_8262_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/7134f88efeca/41598_2022_8262_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/46d303de7c13/41598_2022_8262_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/0210381dbadf/41598_2022_8262_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/301603bf61c8/41598_2022_8262_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/a79fb27fb02d/41598_2022_8262_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/618706689660/41598_2022_8262_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/8cb4969eb98c/41598_2022_8262_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/8e78e7e97022/41598_2022_8262_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/01b0f0b2e37d/41598_2022_8262_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/7134f88efeca/41598_2022_8262_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/46d303de7c13/41598_2022_8262_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/0210381dbadf/41598_2022_8262_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/301603bf61c8/41598_2022_8262_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/a79fb27fb02d/41598_2022_8262_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/618706689660/41598_2022_8262_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/8cb4969eb98c/41598_2022_8262_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/8e78e7e97022/41598_2022_8262_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e71/8924172/01b0f0b2e37d/41598_2022_8262_Fig9_HTML.jpg

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