Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 402, Taiwan, ROC.
Food Microbiol. 2013 Oct;36(1):57-62. doi: 10.1016/j.fm.2013.04.005. Epub 2013 Apr 25.
The microbiological quality of chicken- and pork-based street-food samples from Taichung, Taiwan's night markets (50) and Laguna, Philippines' public places (69) was evaluated in comparison to a microbiological guideline for ready-to-eat foods. Different bacterial contamination patterns were observed between 'hot-grilled' and 'cold cooked/fried' food types from the two sampling locations with 'hot grilled' foods generally showing better microbiological quality. Several samples were found to be unsatisfactory due to high levels of aerobic plate count, coliform, Escherichia coli, and Staphylococcus aureus. The highest counts obtained were 8.2 log cfu g⁻¹, 5.4 log cfu g⁻¹, 4.4 log cfu g⁻¹, and 3.9 log cfu g⁻¹, respectively, suggesting poor food hygiene practices and poor sanitation. Salmonella was found in 8% and 7% of Taichung and Laguna samples, respectively, which made the samples potentially hazardous. None of the samples was found to be positive for Listeria monocytogenes and E. coli O157, but Bacillus cereus was detected at the unsatisfactory level of 4 log cfu g⁻¹ in one Laguna sample. Antimicrobial resistance was observed for Salmonella, E. coli, and S. aureus isolates. Food preparation, cooking, and food handling practices were considered to be contributors to the unacceptable microbiological quality of the street foods. Hence, providing training on food hygiene for the street vendors should result in the improvement of the microbiological quality of street foods. The data obtained in this study can be used as input to microbial risk assessments and in identifying science-based interventions to control the hazards.
对来自台湾台中市夜市(50 个样本)和菲律宾拉古纳市公共场所(69 个样本)的鸡肉和猪肉街头食品样本的微生物质量进行了评估,并与即食食品的微生物学指导方针进行了比较。在这两个采样地点,“热烤”和“冷煮/炸”食品类型之间观察到不同的细菌污染模式,“热烤”食品的微生物质量通常更好。由于需氧平板计数、大肠菌群、大肠杆菌和金黄色葡萄球菌水平较高,一些样本的质量较差。获得的最高计数分别为 8.2logcfug⁻¹、5.4logcfug⁻¹、4.4logcfug⁻¹和 3.9logcfug⁻¹,表明卫生实践和卫生条件较差。沙门氏菌分别在台中市和拉古纳市的 8%和 7%的样本中检出,这使样本具有潜在的危害。未在任何样本中检出李斯特菌和大肠杆菌 O157,但在一个拉古纳样本中检测到蜡样芽胞杆菌,其数量达到 4logcfug⁻¹,不符合标准。对沙门氏菌、大肠杆菌和金黄色葡萄球菌分离株进行了抗药性检测。认为食品制备、烹饪和食品处理方法是导致街头食品微生物质量不可接受的原因。因此,为街头摊贩提供食品卫生培训应能提高街头食品的微生物质量。本研究获得的数据可用于微生物风险评估,并可用于确定基于科学的干预措施,以控制这些危害。