State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, #14 Sec. 3 Renmin Road South, Chengdu, PR China.
Arch Oral Biol. 2013 Sep;58(9):1225-31. doi: 10.1016/j.archoralbio.2013.03.019. Epub 2013 Jun 12.
The local delivery of growth factors such as bone morphogenetic protein-7 (BMP-7) into the tissues around dental implants may improve their osseointegration. We have designed a new method of attaching BMP-7 to a titanium surface and assessed both the retention of the BMP-7 and its effect on osteoblast differentiation.
Adenoviral vector expressing BMP-7 was attached to dental titanium discs by hexon-specific antibodies in a type I collagen-avidin gel. FITC-labelled secondary antibody was used to measure the continuing adherence of the coating after repeated rinsing. Osteoblasts were harvested and seeded on the titanium discs. Gene transduction efficiency and targeting ability were assessed after 24h. Surface morphology was observed by SEM. Cell proliferation and alkaline phosphatase (ALP) activities were measured.
The anti-adenohexon antibody adhered strongly to the collagen-avidin gels. BMP-7 gene expression was localized precisely to cells growing on the gels bound by the hexon-specific antibody. Osteoblasts on the titanium containing Ad-BMP-7 had a higher ALP activity than those without Ad-BMP-7.
This study describes a novel technique for the precise attachment of BMP-7 to titanium surfaces. The process may enhance the osseointegration of dental implants.
将生长因子（如骨形态发生蛋白-7（BMP-7））局部递送到牙种植体周围的组织中可能会改善其骨整合。我们设计了一种将 BMP-7 附着到钛表面的新方法，并评估了 BMP-7 的保留及其对成骨细胞分化的影响。
通过六邻体特异性抗体将表达 BMP-7 的腺病毒载体附着在牙科钛盘上，在 I 型胶原-亲和素凝胶中。使用 FITC 标记的二级抗体来测量经过多次冲洗后涂层的持续粘附。收获成骨细胞并接种在钛盘上。在 24 小时后评估基因转导效率和靶向能力。通过 SEM 观察表面形态。测量细胞增殖和碱性磷酸酶（ALP）活性。
抗腺病毒六邻体抗体强烈附着在胶原-亲和素凝胶上。BMP-7 基因表达精确定位于用六邻体特异性抗体结合的凝胶上生长的细胞。含有 Ad-BMP-7 的钛上的成骨细胞的 ALP 活性高于没有 Ad-BMP-7 的细胞。
本研究描述了一种将 BMP-7 精确附着到钛表面的新技术。该过程可能会增强牙种植体的骨整合。
