多杀巴斯德氏菌毒素膜定位结构域的主链和侧链共振归属
Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin.
作者信息
Brothers Michael C, Geissler Brett, Hisao Grant S, Satchell Karla J F, Wilson Brenda A, Rienstra Chad M
机构信息
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
出版信息
Biomol NMR Assign. 2014 Apr;8(1):221-4. doi: 10.1007/s12104-013-9487-1. Epub 2013 Jun 14.
Abstract
(1)H, (13)C, and (15)N chemical shift assignments are presented for the isolated four-helical bundle membrane localization domain (MLD) from Pasteurella multocida toxin (PMT) in its solution state. We have assigned 99% of all backbone and side-chain carbon atoms, including 99% of all backbone residues excluding proline amide nitrogens. Secondary chemical shift analysis using TALOS+ demonstrates four helices, which align with those observed within the MLD in the crystal structure of the C-terminus of PMT (PDB 2EBF) and confirm the use of the available crystal structures as templates for the isolated MLDs.
摘要
给出了多杀巴斯德氏菌毒素（PMT）的分离四螺旋束膜定位结构域（MLD）在溶液状态下的氢（¹H）、碳（¹³C）和氮（¹⁵N）化学位移归属。我们已归属了所有主链和侧链碳原子的99%，包括除脯氨酸酰胺氮以外所有主链残基的99%。使用TALOS+进行的二级化学位移分析表明有四条螺旋，这与PMT C端晶体结构（PDB 2EBF）中MLD内观察到的螺旋一致，并证实可将现有的晶体结构用作分离MLD的模板。
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本文引用的文献
1
Three-dimensional triple-resonance NMR Spectroscopy of isotopically enriched proteins. 1990.同位素富集蛋白质的三维三共振核磁共振光谱学。1990年。
J Magn Reson. 2011 Dec;213(2):423-41. doi: 10.1016/j.jmr.2011.09.004.
2
Plasma membrane association of three classes of bacterial toxins is mediated by a basic-hydrophobic motif.三类细菌毒素通过碱性-疏水性基序与质膜结合。
Cell Microbiol. 2012 Feb;14(2):286-98. doi: 10.1111/j.1462-5822.2011.01718.x. Epub 2011 Nov 29.
3
Membrane interaction of Pasteurella multocida toxin involves sphingomyelin.多杀巴斯德氏菌毒素的膜相互作用涉及神经鞘磷脂。
FEBS J. 2011 Dec;278(23):4633-48. doi: 10.1111/j.1742-4658.2011.08365.x. Epub 2011 Oct 20.
4
Cellular and molecular action of the mitogenic protein-deamidating toxin from Pasteurella multocida.多杀巴斯德氏菌促分裂原蛋白脱酰胺毒素的细胞和分子作用。
FEBS J. 2011 Dec;278(23):4616-32. doi: 10.1111/j.1742-4658.2011.08158.x. Epub 2011 May 31.
5
Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin.多杀巴斯德氏菌毒素膜靶向 C1 结构域的特性研究。
J Biol Chem. 2010 Aug 13;285(33):25467-75. doi: 10.1074/jbc.M110.102285. Epub 2010 Jun 9.
6
Identification of a conserved membrane localization domain within numerous large bacterial protein toxins.鉴定多种大型细菌蛋白毒素中保守的膜定位结构域。
Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5581-6. doi: 10.1073/pnas.0908700107. Epub 2010 Mar 8.
7
TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts.TALOS+：一种利用核磁共振化学位移预测蛋白质主链扭转角的混合方法。
J Biomol NMR. 2009 Aug;44(4):213-23. doi: 10.1007/s10858-009-9333-z. Epub 2009 Jun 23.
8
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9
Protein production by auto-induction in high density shaking cultures.通过高密度摇瓶培养中的自诱导进行蛋白质生产。
Protein Expr Purif. 2005 May;41(1):207-34. doi: 10.1016/j.pep.2005.01.016.
10
NMRPipe: a multidimensional spectral processing system based on UNIX pipes.NMRPipe：一个基于UNIX管道的多维光谱处理系统。
J Biomol NMR. 1995 Nov;6(3):277-93. doi: 10.1007/BF00197809.
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