Boeszoermenyi Andras, Schmidt Jens C, Cheeseman Iain M, Oberer Monika, Wagner Gerhard, Arthanari Haribabu
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, 02115, USA.
Biomol NMR Assign. 2014 Oct;8(2):275-8. doi: 10.1007/s12104-013-9499-x. Epub 2013 Jun 14.
During mitosis, kinetochores coordinate the attachment of centromeric DNA to the dynamic plus ends of microtubules, which is hypothesized to pull sister chromatids toward opposing poles of the mitotic spindle. The outer kinetochore Ndc80 complex acts synergistically with the Ska (spindle and kinetochore-associated) complex to harness the energy of depolymerizing microtubules and power chromosome movement. The Ska complex is a hexamer consisting of two copies of the proteins Ska1, Ska2 and Ska3, respectively. The C-terminal domain of the spindle and kinetochore-associated protein 1 (Ska1) is the microtubule-binding domain of the Ska complex. We solved the solution structure of the C. elegans microtubule-binding domain (MTBD) of the protein Ska1 using NMR spectroscopy. Here, we report the resonance assignments of the MTBD of C. elegans Ska1.
在有丝分裂过程中，动粒协调着着丝粒DNA与微管动态正端的附着，据推测这会将姐妹染色单体拉向有丝分裂纺锤体的相对两极。外动粒Ndc80复合体与Ska（纺锤体和动粒相关）复合体协同作用，利用微管解聚的能量推动染色体移动。Ska复合体是一个六聚体，分别由两个拷贝的蛋白质Ska1、Ska2和Ska3组成。纺锤体和动粒相关蛋白1（Ska1）的C末端结构域是Ska复合体的微管结合结构域。我们使用核磁共振光谱法解析了秀丽隐杆线虫蛋白质Ska1的微管结合结构域（MTBD）的溶液结构。在此，我们报告了秀丽隐杆线虫Ska1的MTBD的共振归属。
