Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, United States.
Curr Opin Cell Biol. 2012 Feb;24(1):48-56. doi: 10.1016/j.ceb.2011.11.003. Epub 2011 Dec 10.
Successful mitosis depends on the stable, yet regulated attachment of chromosomes to spindle microtubules. The kinetochore, a large macromolecular structure assembled at sites of centromeric heterochromatin, is responsible for generating and regulating these essential attachments. Over the last several years, concerted experimental efforts have brought the structural view of the kinetochore-microtubule interface more clearly into focus. Here, we review important recent advancements and discuss several unresolved questions regarding how kinetochores dynamically bridge mitotic chromosomes to spindle microtubules.
有丝分裂的成功取决于染色体与纺锤体微管稳定而又受到调节的附着。着丝粒是一种在着丝粒异染色质部位组装的大型大分子结构,负责产生和调节这些必需的附着。在过去的几年中,协同的实验努力使着丝粒-微管界面的结构观点更加清晰。在这里,我们回顾了最近的重要进展,并讨论了几个关于着丝粒如何动态连接有丝分裂染色体和纺锤体微管的未解决问题。