Liu Xin, Chen Tong, Hu Yan, Li Kexin, Yan Liushui
Institute of Integrated Chinese and Western Medicine, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.
Biopolymers. 2014 Mar;101(3):210-5. doi: 10.1002/bip.22312.
Sulfated derivatives of polysaccharide from Momordica charantia L. (MCPS) with different degree of sulfation (DS) were synthesized by chlorosulfonic acid method with ionic liquids as solvent. Fourier transform infrared spectra and 13C nuclear magnetic resonance spectra indicated that C-6 substitution was predominant in MCPS compared with the C-2 position. Compared with the native polysaccharide from Momordica charantia L. (MCP), MCPS exhibited more excellent antioxidant activities in vitro, which indicated that sulfated modification could enhance antioxidant activities of MCP. Furthermore, high DS and moderate molecular weight could improve the antioxidant activities of polysaccharide.
以离子液体为溶剂，采用氯磺酸法合成了不同硫酸化程度（DS）的苦瓜多糖硫酸化衍生物（MCPS）。傅里叶变换红外光谱和13C核磁共振光谱表明，与C-2位相比，MCPS中C-6位取代占主导。与天然苦瓜多糖（MCP）相比，MCPS在体外表现出更优异的抗氧化活性，这表明硫酸化修饰可增强MCP的抗氧化活性。此外，高硫酸化程度和适中的分子量可提高多糖的抗氧化活性。
