Ljungdahl Anna, Hanrieder Jörg, Bergquist Jonas, Andersson Malin
Department of Pharmaceutical Bioscience, Drug safety and Toxicology, Uppsala University, Uppsala, Sweden.
Methods Mol Biol. 2013;1023:121-36. doi: 10.1007/978-1-4614-7209-4_7.
Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is one of the most effective tools for localizing small molecules and compounds directly in thin tissue sections. MALDI IMS should be used when the distribution of molecular species is not known and to localize changes due to a disease process or a treatment. In recent years it has become increasingly clear that many pathological processes are not readily correlated to dramatic changes in protein levels. MALDI IMS can aid the localization of areas where the cellular concentration of proteins may be high enough to play an important biological role, but when the precise location is unknown. Here, we present a MALDI IMS protocol and data analysis of molecular imaging of multiple rat brain sections.
基质辅助激光解吸电离（MALDI）成像质谱（IMS）是直接在薄组织切片中定位小分子和化合物的最有效工具之一。当分子种类的分布未知，以及为了定位疾病过程或治疗引起的变化时，应使用MALDI IMS。近年来，越来越明显的是，许多病理过程与蛋白质水平的显著变化并不容易相关。MALDI IMS可以帮助定位蛋白质细胞浓度可能高到足以发挥重要生物学作用但精确位置未知的区域。在这里，我们展示了一个MALDI IMS方案以及对多个大鼠脑切片分子成像的数据分析。
