荧光显微镜作为一种工具，用于阐明COG复合物缺失的哺乳动物细胞中高尔基体糖基转移酶的功能障碍和定位错误。
Fluorescent microscopy as a tool to elucidate dysfunction and mislocalization of Golgi glycosyltransferases in COG complex depleted mammalian cells.
作者信息
Willett Rose A, Pokrovskaya Irina D, Lupashin Vladimir V
机构信息
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
出版信息
Methods Mol Biol. 2013;1022:61-72. doi: 10.1007/978-1-62703-465-4_6.
Abstract
Staining of molecules such as proteins and glycoconjugates allows for an analysis of their localization within the cell and provides insight into their functional status. Glycosyltransferases, a class of enzymes which are responsible for glycosylating host proteins, are mostly localized to the Golgi apparatus, and their localization is maintained in part by a protein vesicular tethering complex, the conserved oligomeric Golgi (COG) complex. Here we detail a combination of fluorescent lectin and immuno-staining in cells depleted of COG complex subunits to examine the status of Golgi glycosyltransferases. The combination of these techniques allows for a detailed characterization of the changes in function and localization of Golgi glycosyltransferases with respect to transient COG subunit depletion.
摘要
对蛋白质和糖缀合物等分子进行染色，有助于分析它们在细胞内的定位，并深入了解其功能状态。糖基转移酶是一类负责对宿主蛋白进行糖基化的酶，大多定位于高尔基体，其定位部分由一种蛋白质囊泡拴系复合体——保守寡聚高尔基体（COG）复合体维持。在此，我们详细介绍了在缺乏COG复合体亚基的细胞中，荧光凝集素和免疫染色相结合的方法，以检测高尔基体糖基转移酶的状态。这些技术的结合能够详细表征高尔基体糖基转移酶在短暂缺乏COG亚基时功能和定位的变化。
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本文引用的文献
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How Golgi glycosylation meets and needs trafficking: the case of the COG complex.高尔基糖基化如何与运输相遇并需要运输：COG 复合物的情况。
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Fatal outcome due to deficiency of subunit 6 of the conserved oligomeric Golgi complex leading to a new type of congenital disorders of glycosylation.由于保守的寡聚高尔基体复合物亚基 6 的缺乏导致的致死性结局，导致一种新型的先天性糖基化障碍。
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Deficiency in COG5 causes a moderate form of congenital disorders of glycosylation.COG5 缺乏导致一种中度形式的先天性糖基化障碍。
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