DeAngelis Paul L
Department of Biochemistry and Molecular Biology, The Oklahoma Center for Medical Glycobiology, The University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Methods Mol Biol. 2013;1022:215-27. doi: 10.1007/978-1-62703-465-4_17.
Multiple glycosyltransferases (GTases) that produce glycosaminoglycans (GAGs) have been identified; -several distinct putative architectures and catalytic abilities have been noted from microbes and vertebrates. Here the preparation and use of a class of enzymes (Class II) from the gram-negative bacterium, Pasteurella multocida, with utility in chemoenzymatic synthesis of GAGs is described. The analyses of sugar products include thin layer chromatography (TLC), matrix-assisted laser desorption ionization mass spectroscopy (MALDI-ToF MS), and polyacrylamide gel electrophoresis (PAGE). The related Chapter 18 is focused on larger molecular weight GAGs analyses as well as the Class I membrane streptococcal and mammalian HA synthases.
已鉴定出多种产生糖胺聚糖（GAGs）的糖基转移酶（GTases）；已从微生物和脊椎动物中发现了几种不同的假定结构和催化能力。本文描述了从革兰氏阴性菌多杀巴斯德氏菌中制备和使用一类酶（II类），其在GAGs的化学酶促合成中具有实用性。糖产物的分析包括薄层色谱法（TLC）、基质辅助激光解吸电离质谱法（MALDI-ToF MS）和聚丙烯酰胺凝胶电泳法（PAGE）。相关的第18章重点介绍了较大分子量GAGs的分析以及I类膜链球菌和哺乳动物透明质酸合酶。
