Zhang H L, Fan H J, Liu X L, Wu Y, Hou S S
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, China.
Genet Mol Res. 2013 May 13;12(2):1582-92. doi: 10.4238/2013.May.13.12.
The perilipin (PLIN) gene is a candidate gene of carcass and fat traits in ducks. In order to study the molecular character of the PLIN gene and its function in different breeds of Chinese ducks, samples were obtained from the Chinese Academy of Agricultural Sciences Research Center for Birds, including 95 Peking ducks of the Z2 series, 91 Peking ducks of the Z4 series, 82 hybrid systems (Z2 x Z4), and 93 Cherry Valley ducks. We used RT-PCR and 3'-RACE to clone the duck PLIN gene, detect SNPs and analyze their associations with carcass and fat traits. A 2212-bp sequence was cloned with the complete coding region and a 3'-untranslated region. We found a nucleotide mutation (C → T) in exon 2 of the PLIN gene. There were no significant correlations between the 3 genotypes (CC, CT, TT) in breast muscle weight (BMW), leg muscle weight (LMW), subcutaneous fat weight (SFW), and intramuscular fat (IMF) in the Cherry Valley duck. The CC and CT genotypes had significant differences in carcass weight (CW), carcass net weight (CNW), and percentage of abdominal fat weight (AFW); there were significant differences in AFW and percentage of SFW. In Z4, there were no significant correlations between the 3 genotypes (TT, CC, and CT) in CW, BMW, LMW, SFW, AFW, the percentage of SFW and AFW, and IMF. CNW was significantly different between TT, CC, and CT genotypes. In Z2 x Z4, there were no significant correlations between the 3 genotypes in CW, BMW, LMW, SFW, AFW, the percentage of SFW and AFW, and IMF, while the CC and CT genotypes had significant differences in CNW. In Z2, there were no significant differences between the 3 genotypes in all traits. We deduced that the PLIN gene is a potential major gene. It is linked to a major gene affecting meat quality traits. This SNP has potential as a molecular marker for marker-assisted selection.
perilipin（PLIN）基因是鸭胴体和脂肪性状的候选基因。为了研究PLIN基因的分子特征及其在中国不同鸭种中的功能，从中国农业科学院家禽研究中心采集样本，包括95只Z2系列北京鸭、91只Z4系列北京鸭、82个杂交组合（Z2×Z4）以及93只樱桃谷鸭。我们采用RT-PCR和3'-RACE技术克隆鸭PLIN基因，检测单核苷酸多态性（SNPs）并分析它们与胴体和脂肪性状的关联。克隆得到一个2212bp的序列，包含完整的编码区和3'非翻译区。我们在PLIN基因的外显子2中发现一个核苷酸突变（C→T）。在樱桃谷鸭中，3种基因型（CC、CT、TT）与胸肌重（BMW）、腿肌重（LMW）、皮下脂肪重（SFW）和肌内脂肪（IMF）之间均无显著相关性。CC和CT基因型在胴体重（CW）、胴体净重（CNW）和腹脂重百分比（AFW）上存在显著差异；在AFW和SFW百分比上也存在显著差异。在Z4中，3种基因型（TT、CC和CT）与CW、BMW、LMW、SFW、AFW、SFW和AFW百分比以及IMF之间均无显著相关性。TT、CC和CT基因型之间的CNW存在显著差异。在Z2×Z4中，3种基因型与CW、BMW、LMW、SFW、AFW、SFW和AFW百分比以及IMF之间均无显著相关性，而CC和CT基因型在CNW上存在显著差异。在Z2中，所有性状的3种基因型之间均无显著差异。我们推断PLIN基因是一个潜在的主基因。它与影响肉质性状的一个主基因连锁。这个SNP有潜力作为分子标记用于标记辅助选择。
