College of Animal Sciences, Zhejiang University, No. 388 Yuhangtang Road, Hangzhou 310058, People's Republic of China.
Mol Biol Rep. 2012 Feb;39(2):1073-7. doi: 10.1007/s11033-011-0833-z. Epub 2011 May 15.
Liver-type fatty acid-binding protein (L-FABP) is a member of intracellular lipid-binding proteins involved in the transportation of fatty acids. We detected the polymorphisms of duck L-FABP gene and its association with the intramuscular fat (IMF) and other fat-related traits. The complete sequence of duck L-FABP gene (four exons and three introns, 2,542 bp) was obtained in this study. The polymorphism of L-FABP gene was examined with direct DNA sequencing method in 231 individuals from different breeds, and a novel single nucleotide polymorphism in the exon 3 was detected. The polymorphism was shown to be associated with the contents of C16:0, C18:3 and the total IMF in pectoral muscle. The content of C16:0 in genotype CC was significantly higher than CT (P < 0.01) and TT (P < 0.01), and the genotype CT was higher than TT (P < 0.01). The content of C18:3 in genotype TT was significantly higher than CC and CT (P < 0.01), whereas the genotype CC and CT had no significant difference (P > 0.05). The content of IMF in genotype CC was significantly higher than CT (P < 0.01). However, no significant difference was detected between genotype CC and TT or genotype CT and TT (P > 0.05).
肝型脂肪酸结合蛋白（L-FABP）是一种参与脂肪酸转运的细胞内脂质结合蛋白。我们检测了鸭 L-FABP 基因的多态性及其与肌内脂肪（IMF）和其他脂肪相关性状的关系。本研究获得了鸭 L-FABP 基因的完整序列（四个外显子和三个内含子，2542bp）。在来自不同品种的 231 个个体中，采用直接 DNA 测序方法检测了 L-FABP 基因的多态性，并在第 3 外显子中检测到了一个新的单核苷酸多态性。该多态性与胸肌中 C16:0、C18:3 和总 IMF 的含量有关。CC 基因型的 C16:0 含量显著高于 CT（P<0.01）和 TT（P<0.01），而 CT 基因型又显著高于 TT（P<0.01）。TT 基因型的 C18:3 含量显著高于 CC 和 CT（P<0.01），而 CC 和 CT 基因型之间没有显著差异（P>0.05）。CC 基因型的 IMF 含量显著高于 CT（P<0.01）。然而，CC 和 TT 基因型或 CT 和 TT 基因型之间没有检测到显著差异（P>0.05）。
