Pavinato V A C, Silva-Brandão K L, Monteiro M, Zucchi M I, Pinheiro J B, Dias F L F, Omoto C
Instituto de Biologia, Universidade Estadual de Campinas, Cidade Universitária Zeferino Vaz, Campinas, SP, Brasil.
Genet Mol Res. 2013 May 14;12(2):1631-5. doi: 10.4238/2013.May.14.3.
We present polymorphic microsatellite markers isolated for genetic studies of the sugarcane borer, Diatraea saccharalis (Fabricius). We isolated 16 microsatellite loci through an enriched genomic library protocol. After characterization, 12 markers showed polymorphic information expressed in the observed number of alleles (ranging from 2 to 7; 5 on average) and in the polymorphism information content (ranging from 0.292 to 0.771; 0.535 on average). These markers can be used in further studies to understand the basic ecological characteristics of the sugarcane borer, e.g., dispersion patterns and population genetic differentiation, associated with distinct geographic scales and host plants.
我们展示了为甘蔗螟虫（Diatraea saccharalis (Fabricius)）的遗传研究分离出的多态微卫星标记。我们通过富集基因组文库方案分离出16个微卫星位点。经过特征分析，12个标记显示出多态信息，表现为观察到的等位基因数量（范围从2到7；平均为5）以及多态信息含量（范围从0.292到0.771；平均为0.535）。这些标记可用于进一步研究，以了解甘蔗螟虫的基本生态特征，例如与不同地理尺度和寄主植物相关的扩散模式和种群遗传分化。
