从中国宁夏回族自治区分离出的一种绵羊痘病毒的鉴定及系统发育分析。

Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China.

作者信息

Zhu X L, Yang F, Li H X, Dou Y X, Meng X L, Li H, Luo X N, Cai X P

机构信息

State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.

出版信息

Genet Mol Res. 2013 May 14;12(2):1670-8. doi: 10.4238/2013.May.14.7.

DOI:10.4238/2013.May.14.7

PMID:23765973

Abstract

An outbreak of sheep pox was investigated in the Ningxia Hui Autonomous Region in China. Through immunofluorescence testing, isolated viruses, polymerase chain reaction identification, and electron microscopic examination, the isolated strain was identified as a sheep pox virus. The virus was identified through sequence and phylogenetic analysis of the P32 gene, open reading frame (ORF) 095, and ORF 103 genes. This study is the first to use the ORF 095 and ORF 103 genes as candidate genes for the analysis of sheep pox. The results showed that the ORF 095 and ORF 103 genes could be used for the genotyping of the sheep pox virus.

摘要

对中国宁夏回族自治区发生的羊痘疫情进行了调查。通过免疫荧光检测、病毒分离、聚合酶链反应鉴定和电子显微镜检查，将分离出的毒株鉴定为羊痘病毒。通过对P32基因、开放阅读框（ORF）095和ORF 103基因进行序列和系统发育分析来鉴定该病毒。本研究首次将ORF 095和ORF 103基因用作分析羊痘的候选基因。结果表明，ORF 095和ORF 103基因可用于羊痘病毒的基因分型。