Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Centro di Referenza Nazionale Controllo Microbiologico e Chimico Molluschi Bivalvi Vivi (CEREM), 60100 Ancona, Italy. Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy. Laboratorio di Patologia Clinica, ASL Latina, Ospedale Santa Maria Goretti, Latina, Italy.
Environ Microbiol Rep. 2010 Feb;2(1):192-7. doi: 10.1111/j.1758-2229.2010.00134.x.
The aims of this study were to investigate the prevalence of total and pathogenic Vibrio parahaemolyticus strains in Italian mussels from different geographical areas of the Adriatic Sea and to determine their serotypes, toxigenic profiles and pandemic potential. Out of 559 samples analysed during 2007, 65 (11.6%) were positive for V. parahaemolyticus. None of the isolates had the genes for thermostable direct haemolysin (tdh) and pandemic marker (toxRS), while five strains (7.7%) had that for TDH-related haemolysin (trh). Regarding geographical location of the toxigenic strains, three were from the Adriatic coast of Puglia, one from Veneto, and one from the Marches. The trh-positive V. parahaemolyticus isolates from Puglia belonged to O1:KUT (2/3) and O1:K37(1/3) serotypes, the trh-positive isolate from the Marches to OUT(O untypeable):KUT serotype, and that from Veneto to O3:KUT. The prevalence of trh-positive V. parahaemolyticus obtained from mussels in this study was higher respect to that reported in previous studies from other European and Extraeuropean countries. The Health Authorities should be more aware of the epidemiological role of environmental V. parahaemolyticus in local food-borne diseases, and increase the microbial surveillance on these microorganisms isolated from shellfish.
本研究的目的是调查来自亚得里亚海不同地理区域的意大利贻贝中总致病性副溶血性弧菌菌株的流行情况，并确定其血清型、产毒谱和大流行潜力。在 2007 年分析的 559 个样本中，有 65 个（11.6%）副溶血性弧菌呈阳性。所有分离株均未携带耐热直接溶血素（tdh）和大流行标志物（toxRS）基因，而 5 株（7.7%）携带 TDH 相关溶血素（trh）基因。关于产毒菌株的地理位置，3 株来自普利亚亚得里亚海沿岸，1 株来自威尼托，1 株来自马尔凯。来自普利亚的 trh 阳性副溶血性弧菌分离株属于 O1:KUT（2/3）和 O1:K37（1/3）血清型，来自马尔凯的 trh 阳性分离株属于 OUT（O 未定型）：KUT 血清型，来自威尼托的 trh 阳性分离株属于 O3:KUT。本研究从贻贝中获得的 trh 阳性副溶血性弧菌的流行率高于以往其他欧洲和欧洲以外国家的研究报告。卫生当局应更加意识到环境副溶血性弧菌在当地食源性疾病中的流行病学作用，并增加对这些从贝类中分离出的微生物的微生物监测。
