"Ecologie des Systèmes Marins Côtiers", UMR 5119 CNRS, IRD, Ifremer, Université Montpellier 2 & 1, Case 093, 34095 Montpellier Cedex 05, France.
Res Microbiol. 2013 Oct;164(8):867-74. doi: 10.1016/j.resmic.2013.06.005. Epub 2013 Jun 12.
Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 × 10³ MPN/l, 0.09 to 1.1 × 10³ MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were present in water, sediment and shellfish samples collected from these lagoons. The number of V. vulnificus in water, sediment and shellfish samples ranged from 1 to 1.1 × 10³ MPN/l, 0.07 to 110 MPN/ml and 0.04 to 15 MPN/g, respectively, during summer. V. vulnificus was not detected during winter. V. cholerae was rarely detected in water and sediment during summer. In summary, results of this study highlight the finding that the three human pathogenic Vibrio spp. are present in the lagoons and constitute a potential public health hazard.
副溶血性弧菌、创伤弧菌和霍乱弧菌都是人类病原体。关于这些法国沿海泻湖中的弧菌，人们知之甚少。本研究的目的是使用最可能数-聚合酶链反应（MPN-PCR）调查法国三个地中海沿海泻湖水中、贝类和沉积物中这些弧菌的发生率。在夏季，从三个泻湖采集的水中、沉积物、贻贝和蛤中的副溶血性弧菌总数从 1 到>1.1×10³ MPN/l、0.09 到 1.1×10³ MPN/ml、9 到 210 MPN/g 和 1.5 到 2.1 MPN/g 不等。在冬季，除贻贝外，所有样本均含有副溶血性弧菌，但浓度非常低。这些泻湖的水样、沉积物和贝类样本中存在致病性（tdh-或 trh2-阳性）副溶血性弧菌。在夏季，水中、沉积物和贝类样本中的创伤弧菌数量从 1 到 1.1×10³ MPN/l、0.07 到 110 MPN/ml 和 0.04 到 15 MPN/g 不等。冬季未检测到创伤弧菌。霍乱弧菌在夏季水中和沉积物中很少被检测到。总之，本研究结果强调了以下发现，即三种人类致病性弧菌存在于泻湖中，构成了潜在的公共卫生危害。
