副溶血弧菌经 UVC 或 UVA 照射后的应激反应差异。

Differences in stress response after UVC or UVA irradiation in Vibrio parahaemolyticus.

机构信息

Departments of Preventive Environment and Nutrition, and Nutrition and Metabolism, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto-cho, Tokushima, Tokushima, Japan. Department of Electrical and Electronic Engineering, Institute of Socio Techno Sciences, The University of Tokushima Graduate School, Minamijosanjima-cho, Tokushima, Tokushima, Japan.

出版信息

Environ Microbiol Rep. 2010 Oct;2(5):660-6. doi: 10.1111/j.1758-2229.2010.00154.x.

DOI:10.1111/j.1758-2229.2010.00154.x

PMID:23766253

Abstract

The SOS response is a global regulatory network for repairing DNA damage induced by various environmental stresses such as UV irradiation. The Escherichia coli SOS response has been extensively studied. However, there are no reports on the SOS response in Vibrio parahaemolyticus. In this study, we examined the SOS response in V. parahaemolyticus and compared the differential expression of genes induced by UVC and UVA irradiation. In UVC-exposed wild-type cells, expression of several DNA repair genes was increased. However, expression of these genes was not increased in ΔrecA or lexA mutants. Cell filamentation was observed in wild-type cells, but not in ΔrecA and lexA mutant cells. Sensitivity to UVC was significantly increased in ΔrecA, lexA mutant and Δlon strains compared with wild type. In the case of UVA irradiation, LexA-controlled DNA repair genes were minimally induced and cell filamentation was not observed. Sensitivity to UVA was the same in the mutant and wild-type strains. These findings suggest that there is a RecA-LexA-mediated SOS response in V. parahaemolyticus, and that this response is important to UVC tolerance but does not contribute to UVA tolerance.

摘要

SOS 响应是一个用于修复由各种环境应激（如紫外线照射）引起的 DNA 损伤的全局调控网络。大肠杆菌 SOS 响应已得到广泛研究。然而，在副溶血性弧菌中尚未有关于 SOS 响应的报道。在本研究中，我们检测了副溶血性弧菌中的 SOS 响应，并比较了 UVC 和 UVA 照射诱导的基因差异表达。在 UVC 暴露的野生型细胞中，几种 DNA 修复基因的表达增加。然而，在 recA 或 lexA 突变体中，这些基因的表达并未增加。在野生型细胞中观察到细胞丝状化，但在 recA 和 lexA 突变体细胞中未观察到。与野生型相比，recA、lexA 突变体和 lon 缺失菌株对 UVC 的敏感性显著增加。在 UVA 照射的情况下，LexA 控制的 DNA 修复基因的诱导最小，且未观察到细胞丝状化。突变体和野生型菌株对 UVA 的敏感性相同。这些发现表明，副溶血性弧菌中存在 RecA-LexA 介导的 SOS 响应，该响应对 UVC 耐受很重要，但对 UVA 耐受没有贡献。