Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
Environ Microbiol Rep. 2010 Dec;2(6):770-4. doi: 10.1111/j.1758-2229.2010.00183.x.
Gingivitis and dental caries are two of the most predominant diseases in humans. Both conditions are easily treated with the removal of the plaque biofilm by brushing or the use of oral hygiene products. In both cases, pathogenic taxa found within the plaque biofilm are the causal agents of the disease. Actinomyces naeslundii, Fusobacterium nucleatum and Prevotalla intermedia have all been implicated in the development of gingivitis, while Streptococcus mutans is the main organism associated with dental caries. Many studies have so far focused on the use of culture methods to detect and enumerate the pathogenic taxa within plaque samples. However, these methods are both labour intensive and biased towards culturable taxa. In the present study, a novel high-throughput multi-triplex quantitative PCR method was developed with the aim to investigate the community dynamics associated with oral communities. Three triplex assays were designed targeting taxa associated with gingivitis and dental caries as well as oral health. Saliva samples collected from healthy individuals were used in order to validate the newly developed method.
牙龈炎和龋齿是人类最常见的两种疾病。通过刷牙或使用口腔卫生产品去除菌斑生物膜，这两种疾病都很容易治疗。在这两种情况下，菌斑生物膜中发现的病原分类群是疾病的致病因子。内氏放线菌、核梭杆菌和中间普雷沃菌都与牙龈炎的发展有关，而变形链球菌是与龋齿相关的主要生物体。迄今为止，许多研究都集中在使用培养方法来检测和计数菌斑样本中的病原分类群。然而，这些方法既费时费力，又偏向于可培养的分类群。本研究开发了一种新的高通量多重定量 PCR 方法，旨在研究与口腔群落相关的群落动态。设计了三个三重检测，针对与牙龈炎和龋齿以及口腔健康相关的分类群。使用从健康个体收集的唾液样本对新开发的方法进行了验证。
