Academic Centre for Dentistry Amsterdam, Preventive Dentistry, Amsterdam, The Netherlands.
Netherlands Organisation for applied scientific research (TNO), Microbiology and Systems Biology Zeist, The Netherlands.
Eur J Oral Sci. 2022 Apr;130(2):e12858. doi: 10.1111/eos.12858. Epub 2022 Feb 26.
In general, saliva is used for microbiota analysis in longitudinal studies, and several collection methods are being used. Using a robust sample collection procedure is important, as it may influence salivary composition. This study explored the comparability of the microbiota of swabbed and spit saliva. Twenty-two females participated in this cross-sectional study. The bacterial composition of the three saliva samples (swab collected by the participant (SW-P), swab collected by the researcher (SW-R), and spit (SP) was assessed by 16S rRNA gene amplicon sequencing. The bacterial composition of the swabbed and the spit saliva was significantly different irrespective of the operator, and Shannon diversity was significantly higher in spit saliva than in SW-P and SW-R. The salivary microbiota of spit and swabbed adult saliva differs significantly. Research on microbial composition therefore requires collection of similar saliva sample types in all study participants.
一般来说，唾液用于纵向研究中的微生物组分析，并且正在使用几种收集方法。使用稳健的样本收集程序很重要，因为它可能会影响唾液成分。本研究探讨了擦拭和吐口水的唾液微生物组的可比性。22 名女性参与了这项横断面研究。通过 16S rRNA 基因扩增子测序评估了三种唾液样本（参与者收集的拭子（SW-P）、研究人员收集的拭子（SW-R）和吐口水（SP）的细菌组成。无论操作者如何，擦拭和吐口水的细菌组成都有显著差异，并且与 SW-P 和 SW-R 相比，吐口水的香农多样性显著更高。成人唾液的唾液微生物组差异显著。因此，关于微生物组成的研究需要在所有研究参与者中收集类似的唾液样本类型。
