Sinno Hani, Malhotra Meenakshi, Lutfy Justyn, Jardin Barbara, Winocour Sebastian, Brimo Fadi, Beckman Lorne, Watters Kevin, Philip Anie, Williams Bruce, Prakash Satya
Division of Plastic and Reconstructive Surgery, Department of Surgery, McGill University, Montreal, QC, Canada H3G 1A4 ; Biomedical Technology and CellTherapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 Rue University, Room 311, Lyman Duff Medical Building, Montreal, QC, Canada H3A 2B4.
Plast Surg Int. 2013;2013:243853. doi: 10.1155/2013/243853. Epub 2013 May 23.
Background. Complements C3 and C5 have independently been shown to augment and increase wound healing and strength. Our goal was to investigate the combinatorial effect of complements C3 and C5 on wound healing. Methods. Each rat served as its own control where topical collagen was applied to one incision and 100 nM of C3 and C5 in collagen vehicle was applied to the other incision (n = 6). To compare between systemic effects, a sham group of rats (n = 6) was treated with collagen alone on one wound and saline on the other. At day 3, the tissue was examined for maximal breaking strength (MBS) and sectioned for histological examination. Results. There was a statistically significant 88% increase in MBS with the topical application of C3C5 when compared to sham wounds (n < 0.05). This was correlated with increased fibroblast and collagen deposition in the treated wounds. Furthermore, there appeared to be an additive hemostatic effect with the C3C5 combination. Conclusions. The combination of complements C3 and C5 as a topical application drug to skin wounds significantly increased wound healing maximum breaking strength as early as 3 days.
背景。补体C3和C5已被独立证明可促进并增强伤口愈合及强度。我们的目标是研究补体C3和C5对伤口愈合的联合作用。方法。每只大鼠均作为自身对照，在一个切口处局部应用胶原蛋白，在另一个切口处应用含100 nM C3和C5的胶原蛋白载体（n = 6）。为比较全身效应，一组假手术大鼠（n = 6）在一个伤口处单独应用胶原蛋白，在另一个伤口处应用生理盐水。在第3天，检查组织的最大断裂强度（MBS）并切片进行组织学检查。结果。与假手术伤口相比，局部应用C3C5时MBS有统计学意义的88%增加（n < 0.05）。这与处理伤口中增加的成纤维细胞和胶原蛋白沉积相关。此外，C3C5组合似乎有相加的止血作用。结论。补体C3和C5组合作为皮肤伤口的局部应用药物，早在3天时就显著增加了伤口愈合的最大断裂强度。
