Montreal, Quebec, Canada From the Divisions of Plastic Surgery, Department of Surgery, and the Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, the Department of Pathology, and the Orthopedic Research Laboratory, McGill University.
Plast Reconstr Surg. 2012 Sep;130(3):523-529. doi: 10.1097/PRS.0b013e31825dc02d.
Delayed-healing traumatic, surgical, and chronic wounds can be detrimental to patients and the health care system. The authors set out to investigate the effects of complement C5, a naturally occurring chemotactic cytokine, on wounds.
The authors examined the effects of complement C5 on the rat paired skin incision model. Each rat served as its own control where topical collagen was applied to one incision and 100 nM of C5 in collagen vehicle was applied to the other incision. Rats were killed on days 3 (n = 6), 7 (n = 6), and 28 (n = 5) after wounding.
There was a statistically significant, 65 percent increase in maximum wound breaking strength with the topical application of C5 at day 3 (p < 0.01). The increase persisted to 14 percent at 7 days after wounding (p < 0.05). When compared with the sham group, the C5-treated wound strength increased by 83 percent at day 3 and 64 percent at day 7. There was no change in breaking strength at 28 days. Western blot analysis demonstrated a significant increase in collagen and fibronectin content in the C5-treated wounds.
Topical application of C5 to skin wounds significantly increases wound healing maximum breaking strength as early as 3 days and up to 7 days after wounding. C5 accelerated wound healing by at least 4 days in the first week of wounding. This was correlated with an increase in vascular permeability, increased inflammatory cell recruitment, subsequent fibroblast migration, and increased collagen deposition.
延迟愈合的创伤性、手术性和慢性伤口会对患者和医疗保健系统造成损害。作者着手研究补体 C5（一种天然存在的趋化细胞因子）对伤口的影响。
作者研究了补体 C5 对大鼠双侧皮肤切开模型的影响。每只大鼠自身对照，其中一个切口应用胶原，另一个切口应用胶原载体中的 100 nM C5。大鼠在创伤后第 3 天（n = 6）、第 7 天（n = 6）和第 28 天（n = 5）处死。
在第 3 天，局部应用 C5 可使最大伤口断裂强度显著增加 65%（p < 0.01）。在创伤后 7 天，这一增长持续了 14%（p < 0.05）。与假手术组相比，C5 治疗组的伤口强度在第 3 天增加了 83%，在第 7 天增加了 64%。在第 28 天，断裂强度没有变化。Western blot 分析显示，C5 处理的伤口中胶原和纤维连接蛋白含量显著增加。
在创伤后 3 天和 7 天内，将 C5 局部应用于皮肤伤口可显著增加伤口愈合的最大断裂强度。在创伤的第一周，C5 至少将伤口愈合提前了 4 天。这与血管通透性增加、炎症细胞募集增加、随后的成纤维细胞迁移以及胶原沉积增加有关。
