局部应用胶原蛋白治疗对大鼠伤口抗张强度和瘢痕美容效果的影响。
The effects of topical collagen treatment on wound breaking strength and scar cosmesis in rats.
作者信息
Sinno Hani, Malhotra Meenakshi, Lutfy Justyn, Jardin Barbara, Winocour Sebastian, Brimo Fadi, Beckman Lorne, Watters Kevin, Philip Anie, Williams Bruce, Prakash Satya
机构信息
Division of Plastic and Reconstructive Surgery, Department of Surgery; ; Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine;
出版信息
Can J Plast Surg. 2012 Fall;20(3):181-5. doi: 10.1177/229255031202000318.
BACKGROUND
Topical application of collagen has been suggested to enhance wound healing; however, its long-term effect on wounds has not been studied in a rat model.
HYPOTHESIS
Topical application of collagen type I will not facilitate incision healing or cosmesis in rats up to 28 days postwounding.
METHODS
The effects of bovine collagen type I (6 mg/mL) on the rat surgical paired skin incision model were examined. Each rat served as its own control in which topical collagen was applied to one incision while normal saline (0.9%) was applied to the other incision. Rats were euthanized three (n=6), seven (n=6) and 28 (n=5) days after wounding. Tissue harvested from each time point was examined for maximal breaking strength, and for biochemical and histological analysis.
RESULTS
There were no statistically significant differences (ie, P<0.05) in maximum wound breaking strength between the collagen- and saline-treated wounds at all time points. Histological analysis revealed a similar infiltration of inflammatory cells and fibroblasts in the wound edges of all incisions when matched with time of wounding. Western blot analysis revealed no differences in fibronectin or collagen I content in all wounds in each rat.
CONCLUSIONS
The topical application of collagen did not facilitate wound healing from three to 28 days in the rat wound model.
背景
已有研究表明，局部应用胶原蛋白可促进伤口愈合；然而，其对伤口的长期影响尚未在大鼠模型中进行研究。
假设
在大鼠受伤后长达28天内，局部应用I型胶原蛋白不会促进切口愈合或改善美观。
方法
检测了牛I型胶原蛋白（6mg/mL）对大鼠手术配对皮肤切口模型的影响。每只大鼠均作为自身对照，其中一个切口局部应用胶原蛋白，另一个切口局部应用生理盐水（0.9%）。在受伤后3天（n=6）、7天（n=6）和28天（n=5）对大鼠实施安乐死。对每个时间点采集的组织进行最大断裂强度检测以及生化和组织学分析。
结果
在所有时间点，胶原蛋白处理组伤口与生理盐水处理组伤口之间的最大伤口断裂强度均无统计学显著差异（即P<0.05）。组织学分析显示，与受伤时间匹配时，所有切口伤口边缘的炎性细胞和成纤维细胞浸润情况相似。蛋白质印迹分析显示，每只大鼠所有伤口中的纤连蛋白或I型胶原蛋白含量均无差异。
结论
在大鼠伤口模型中，局部应用胶原蛋白在受伤后3至28天内并未促进伤口愈合。
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