Benesh Deann L, Crowley Erin S, Bird Patrick M
3M Food Safety, 3M Center, Bldg 260-6B-01, Saint Paul, MN 55144-1000, USA.
J AOAC Int. 2013 Mar-Apr;96(2):225-8. doi: 10.5740/jaoacint.govval02.
A validation study of the 3M Petrifilm Environmental Listeria (EL) Plate (3M Food Safety, St. Paul, MN) was conducted at Q Laboratories, Inc., Cincinnati, OH. The method was compared to the Health Canada MFHPB-30 reference method for the analysis of stainless steel environmental surfaces. Twenty replicates of the environmental surface were analyzed at a low and a high inoculum level. The low-level sampling area was inoculated with 0.2-2 CFU/5 cm2, and the high-level sampling area was inoculated with 2-5 CFU/5 cm2. Five control replicates were also analyzed at 0 CFU/5 cm2. There was no significant difference in the number of positives detected by the 3M Petrifilm EL Plate method and the Health Canada MFHPB-30 reference method for the environmental surface analyzed in this study.
俄亥俄州辛辛那提市的Q实验室对3M Petrifilm环境李斯特菌（EL）检测平板（3M食品安全公司，明尼苏达州圣保罗）进行了验证研究。该方法与加拿大卫生部MFHPB - 30参考方法用于分析不锈钢环境表面进行了比较。在低接种量和高接种量水平下对环境表面的20个重复样本进行了分析。低水平采样区域接种量为0.2 - 2 CFU/5 cm²，高水平采样区域接种量为2 - 5 CFU/5 cm²。还对5个0 CFU/5 cm²的对照重复样本进行了分析。在本研究分析的环境表面上，3M Petrifilm EL检测平板法和加拿大卫生部MFHPB - 30参考方法检测到的阳性数量没有显著差异。
