Johnson Ronald, Mills John
bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 63042, USA.
J AOAC Int. 2013 Mar-Apr;96(2):242-5. doi: 10.5740/jaoacint.govval04.
The AOAC GovVal study compared the VIDAS Listeria species Xpress (LSX) to the Health Products and Food Branch MFHPB-30 reference method for detection of Listeria on stainless steel. The LSX method utilizes a novel and proprietary enrichment media, Listeria Xpress broth, enabling detection of Listeria species in environmental samples with the automated VIDAS in a minimum of 26 h. The LSX method also includes the use of the chromogenic media, chromID Ottaviani Agosti Agar (OAA) and chromID Lmono for confirmation of LSX presumptive results. In previous AOAC validation studies comparing VIDAS LSX to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA-BAM) and the U.S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) reference methods, the LSX method was approved as AOAC Official Method 2010.02 for the detection of Listeria species in dairy products, vegetables, seafood, raw meats and poultry, and processed meats and poultry, and as AOAC Performance Tested Method 100501 in a variety of foods and on environmental surfaces. The GovVal comparative study included 20 replicate test portions each at two contamination levels for stainless steel where fractionally positive results (5-15 positive results/20 replicate portions tested) were obtained by at least one method at one level. Five uncontaminated controls were included. In the stainless steel artificially contaminated surface study, there were 25 confirmed positives by the VIDAS LSX assay and 22 confirmed positives by the standard culture methods. Chi-square analysis indicated no statistical differences between the VIDAS LSX method and the MFHPB-30 standard methods at the 5% level of significance. Confirmation of presumptive LSX results with the chromogenic OAA and Lmono media was shown to be equivalent to the appropriate reference method agars. The data in this study demonstrate that the VIDAS LSX method is an acceptable alternative method to the MFHPB-30 standard culture method for the detection of Listeria species on stainless steel.
美国官方分析化学师协会（AOAC）的GovVal研究将VIDAS李斯特菌属快速检测法（LSX）与加拿大卫生部健康产品和食品处的MFHPB - 30参考方法进行了比较，以检测不锈钢表面的李斯特菌。LSX方法使用了一种新型的专利富集培养基——李斯特菌快速肉汤，能够通过自动化的VIDAS在至少26小时内检测环境样品中的李斯特菌属。LSX方法还包括使用显色培养基chromID Ottaviani Agosti琼脂（OAA）和chromID Lmono来确认LSX的初步检测结果。在之前美国官方分析化学师协会的验证研究中，将VIDAS LSX与美国食品药品监督管理局的《细菌学分析手册》（FDA - BAM）以及美国农业部食品安全与检验局（USDA - FSIS）的参考方法进行了比较，LSX方法被批准为美国官方分析化学师协会的2010.02官方方法，用于检测乳制品、蔬菜、海鲜、生肉和家禽以及加工肉和家禽中的李斯特菌属，并且在各种食品和环境表面作为美国官方分析化学师协会性能测试方法100501。GovVal比较研究包括在不锈钢的两个污染水平下各20个重复测试部分，其中至少有一种方法在一个水平上获得了部分阳性结果（每20个重复部分中有5 - 15个阳性结果）。还包括5个未受污染的对照。在不锈钢人工污染表面研究中，VIDAS LSX检测法有25个确认阳性结果，标准培养方法有22个确认阳性结果。卡方分析表明，在5%的显著性水平下，VIDAS LSX方法与MFHPB - 30标准方法之间没有统计学差异。用显色OAA和Lmono培养基确认LSX初步结果被证明等同于适当的参考方法琼脂。本研究中的数据表明，VIDAS LSX方法是检测不锈钢表面李斯特菌属的一种可接受的替代方法，可替代MFHPB - 30标准培养方法。
