Benesh Deann L, Crowley Erin S, Bird Patrick M
3M Food Safety, 3M Center, Bldg 260-6B-01, Saint Paul, MN 55144-1000, USA.
J AOAC Int. 2013 Mar-Apr;96(2):218-24. doi: 10.5740/jaoacint.govval01.
A validation study of the 3M Tecra Listeria Visual Immunoassay (VIA; 3M Food Safety, St. Paul, MN) was conducted at Q Laboratories, Inc., Cincinnati, OH. The 3M Tecra Listeria VIA method was compared to the Health Canada MFHPB-30 reference method for the analysis of five ready-to-eat (RTE) meats: deli turkey, hot dogs, liver pate, raw fermented sausage, and deli ham, and on a stainless steel environmental surface. Twenty replicates of each of the five food matrixes were analyzed at a low and a high inoculum level. The low-level test portions were inoculated with 0.2-2 CFU/25 g, and the high-level test portions with 2-5 CFU/25 g. In addition, 20 replicates of one environmental surface were analyzed at a low and a high inoculum level. The low-level sampling area was inoculated with 0.2-2 CFU/5 cm2, and the high-level area with 2-5 CFU/5 cm2. Five control replicates were also analyzed at 0 CFU/25 g (uninoculated) for the foods and at 0 CFU/5 cm2 for the environmental sampling area. There was no significant difference in the number of positives detected by the 3M Tecra Listeria VIA and the Health Canada MFHPB-30 reference method for four of the RTE meats and the stainless steel environmental surface analyzed in this study. For the raw, fermented sausage, there was a significant difference in the number of positives detected for the high inoculum level by the 3M Tecra Listeria VIA and the Health Canada MFHPB-30 reference method, with the 3M Tecra Listeria VIA method detecting more positives.
俄亥俄州辛辛那提市的Q实验室对3M Tecra李斯特菌可视化免疫测定法（VIA；3M食品安全公司，明尼苏达州圣保罗）进行了验证研究。将3M Tecra李斯特菌VIA方法与加拿大卫生部MFHPB - 30参考方法进行比较，以分析五种即食（RTE）肉类：熟食火鸡、热狗、肝酱、生发酵香肠和熟食火腿，以及不锈钢环境表面。对五种食品基质中的每一种进行20次重复分析，接种水平分为低水平和高水平。低水平测试部分接种0.2 - 2 CFU/25 g，高水平测试部分接种2 - 5 CFU/25 g。此外，对一个环境表面进行20次重复分析，接种水平分为低水平和高水平。低水平采样区域接种0.2 - 2 CFU/5 cm²，高水平区域接种2 - 5 CFU/5 cm²。还对食品在0 CFU/25 g（未接种）时进行了五次对照重复分析，对环境采样区域在0 CFU/5 cm²时进行了分析。在本研究中分析的四种即食肉类和不锈钢环境表面中，3M Tecra李斯特菌VIA检测到的阳性数量与加拿大卫生部MFHPB - 30参考方法之间没有显著差异。对于生发酵香肠，3M Tecra李斯特菌VIA方法和加拿大卫生部MFHPB - 30参考方法在高水平接种时检测到的阳性数量存在显著差异，3M Tecra李斯特菌VIA方法检测到的阳性更多。
