Johnson Ronald, Mills John
bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 63042, USA.
J AOAC Int. 2013 Mar-Apr;96(2):246-50. doi: 10.5740/jaoacint.govval05.
This AOAC GovVal study compared the VIDAS Listeria monocytogenes II (LMO2) to the Health Products and Food Branch MFHPB-30 reference method for detection of L. monocytogenes in ready-to-eat (RTE) meats. The VIDAS LMO2 test is an automated enzyme-linked fluorescent immunoassay for the detection of L. monocytogenes in foods. The LMO2 test, following the enrichment procedure from the MFLP-33 method, also included use of the chromogenic media, chromID Ottaviani Agosti Agar (OAA) and chromID Lmono for confirmation of LMO2 presumptive results. In previous AOAC validation studies comparing the VIDAS LMO2 method to the U.S. Food and Drug Administration Bacteriological Analytical Manual and U.S. Department of Agriculture-Food Safety and Inspection Service reference methods, LMO2 was approved as AOAC Official Method 2004.02 for the detection of L. monocytogenes in dairy products, vegetables, seafood, raw meats and poultry, and processed meats and poultry. The GovVal comparative study included 20 replicate test portions, each at two contamination levels for each matrix, where fractionally positive results (5-15 positive results/20 replicate portions tested) were obtained by at least one method at one level. Five uncontaminated controls were included. Chi-square analysis of the comparative data in this study indicates no statistical differences between the VIDAS LMO2 and the MFHPB-30 standard methods at the 5% level of significance. Confirmation of presumptive LMO2 results with the chromogenic OAA and Lmono media was shown to be equivalent to the appropriate reference method agars. The data demonstrate that the VIDAS LMO2 method is an acceptable alternative method to the MFHPB-30 standard culture method for the detection of L. monocytogenes in RTE meats, including liver paté, hot dogs, raw fermented sausage, sliced deli turkey, and sliced deli ham.
这项AOAC GovVal研究将VIDAS单核细胞增生李斯特菌II检测法（LMO2）与加拿大卫生部健康产品和食品处的MFHPB - 30参考方法进行了比较，以检测即食（RTE）肉类中的单核细胞增生李斯特菌。VIDAS LMO2检测是一种用于检测食品中单核细胞增生李斯特菌的自动化酶联荧光免疫分析方法。LMO2检测在采用MFLP - 33方法的富集程序之后，还使用了显色培养基chromID奥塔维亚尼 - 阿戈斯蒂琼脂（OAA）和chromID Lmono来确认LMO2的初步检测结果。在之前将VIDAS LMO2方法与美国食品药品监督管理局《细菌学分析手册》以及美国农业部食品安全与检验局参考方法进行比较的AOAC验证研究中，LMO2被批准为AOAC官方方法2004.02，用于检测乳制品、蔬菜、海鲜、生肉和家禽以及加工肉类和家禽中的单核细胞增生李斯特菌。GovVal比较研究包括20个重复检测样本，每种基质的每个污染水平各有20个，其中至少有一种方法在一个水平上获得了部分阳性结果（每20个重复样本中有5 - 15个阳性结果）。研究还包括5个未受污染的对照样本。本研究中比较数据的卡方分析表明，在5%的显著性水平下，VIDAS LMO2和MFHPB - 30标准方法之间没有统计学差异。结果表明，用显色OAA和Lmono培养基确认LMO2初步检测结果与相应的参考方法琼脂等效。数据表明，对于检测即食肉类（包括肝泥、热狗、生发酵香肠、切片熟食火鸡和切片熟食火腿）中的单核细胞增生李斯特菌，VIDAS LMO2方法是MFHPB - 30标准培养方法的一种可接受的替代方法。
