Curiale M S, Lepper W, Robison B
Silliker Laboratories Group, Inc., Chicago Heights, IL 60411.
J AOAC Int. 1994 Nov-Dec;77(6):1472-89.
A collaborative study was conducted to evaluate Listeria-Tek, an enzyme-linked immunosorbent assay (ELISA) for detection of Listeria monocytogenes and other Listeria spp. in foods. The present ELISA method was compared to the U.S. Food and Drug Administration culture method for detection of L. monocytogenes in dairy products and seafoods and to the U.S. Department of Agriculture Food Safety and Inspection Service method for detection of L. monocytogenes in meats. Replicate samples of 6 food types (frankfurters, roast beef, Brie cheese, 2% milk, raw shrimp, and crab meat) inoculated with L. monocytogenes and uninoculated control samples were analyzed by the collaborators. L. monocytogenes was identified in 593 samples by the ELISA method and in 574 samples using culture procedures. Identical results were obtained for 506 positive samples and 419 negative samples using the ELISA and culture methods for an overall agreement rate of 85.6%. The enzyme-linked immunoassay for detection of L. monocytogenes in dairy, seafood, and meat products has been adopted first action by AOAC INTERNATIONAL.
开展了一项合作研究，以评估用于检测食品中单核细胞增生李斯特菌及其他李斯特菌属的酶联免疫吸附测定法（ELISA）——Listeria-Tek。将目前的ELISA方法与美国食品药品监督管理局检测乳制品和海产品中单核细胞增生李斯特菌的培养方法，以及美国农业部食品安全检验局检测肉类中单核细胞增生李斯特菌的方法进行了比较。参与合作的人员对接种了单核细胞增生李斯特菌的6种食品类型（法兰克福香肠、烤牛肉、布里干酪、2%牛奶、生虾和蟹肉）的重复样本以及未接种的对照样本进行了分析。通过ELISA方法在593个样本中鉴定出了单核细胞增生李斯特菌，使用培养程序在574个样本中鉴定出了该菌。使用ELISA和培养方法对506个阳性样本和419个阴性样本获得了相同的结果，总体一致率为85.6%。用于检测乳制品、海产品和肉类产品中单核细胞增生李斯特菌的酶联免疫测定法已被美国官方分析化学师协会首次采用。
