a Department of Pharmacognosy , Cairo University , Giza , Egypt.
Nat Prod Res. 2013;27(23):2224-6. doi: 10.1080/14786419.2013.805333. Epub 2013 Jun 14.
Chemical investigation of an ethyl acetate soluble fraction of Sinularia polydactyla (Ehrenberg) led to the isolation of three known terpenoides, two of them sterols, 24-methylcholestane-3β,5α,6β,25-tetrol 25-monoacetate (1), 24-methylcholestane-5-en-3β,25-diol (2), in addition to a cembranoid diterpene, durumolide C (3), for the first time. The cytotoxicity and antimicrobial activities of the ethyl acetate extract and the isolated compounds 1-3 were evaluated in vitro. Durumolide C (3) showed selective cytotoxicity against HepG2 (IC50 1.0 μg/mL), whereas 24-methylcholestane-3β,5α,6β,25-tetrol 25-monoacetate (1) showed IC50 of 6.1 and 8.2 μg/mL against Hep2 and HCT human cancer cell lines, respectively.
从鹿角珊瑚（Ehrenberg）的乙酸乙酯可溶部分进行化学研究，分离得到三种已知的萜类化合物，其中两种为甾醇，分别为 24-甲基胆甾烷-3β，5α，6β，25-四醇 25-单乙酸酯（1）和 24-甲基胆甾烷-5-烯-3β，25-二醇（2），此外还有一种倍半萜二萜类化合物，杜罗莫利德 C（3），这是首次从该种珊瑚中分离得到。评估了乙酸乙酯提取物和分离得到的化合物 1-3 的体外细胞毒性和抗菌活性。杜罗莫利德 C（3）对 HepG2 具有选择性细胞毒性（IC50 为 1.0 μg/mL），而 24-甲基胆甾烷-3β，5α，6β，25-四醇 25-单乙酸酯（1）对 Hep2 和 HCT 人癌细胞系的 IC50 分别为 6.1 和 8.2 μg/mL。
