使用荧光氨羟试剂选择性检测 DNA 中的 5-甲酰基-2'-脱氧胞苷。
Selective detection of 5-formyl-2'-deoxycytidine in DNA using a fluorogenic hydroxylamine reagent.
机构信息
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. of China.
出版信息
Org Lett. 2013 Jul 5;15(13):3266-9. doi: 10.1021/ol401290d. Epub 2013 Jun 14.
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
荧光羟胺试剂被用于通过标记途径检测 5-fC。通过化学合成、HPLC、变性 PAGE 和 DNA MS 来验证该探针与 5-fC 发生反应，具有寡脱氧核苷酸选择性，从而方便、定量地实现荧光法检测 5-fC。还研究了在基因组中荧光检测 5-fC 的可行性。
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